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Effects of stocking density on lipid deposition and expression of lipid-related genes in Amur sturgeon (Acipenser schrenckii)

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Abstract

To investigate the correlation between lipid deposition variation and stocking density in Amur sturgeon (Acipenser schrenckii) and the possible physiological mechanism, fish were conducted in different stocking densities (LSD 5.5 kg/m3, MSD 8.0 kg/m3, and HSD 11.0 kg/m3) for 70 days and then the growth index, lipid content, lipase activities, and the mRNA expressions of lipid-related genes were examined. Results showed that fish subjected to higher stocking density presented lower final body weights (FBW), specific growth ratio (SGR), and gonad adipose tissue index (GAI) (P < 0.05). Lower lipid content was observed in the liver, gonad adipose tissue and muscle in sturgeons held in HSD group (P < 0.05). The serum concentrations of triglyceride (TG), total cholesterol (TC), and high-density lipoprotein cholesterol (HDL-C) decreased significantly with increasing stocking density, while no significant change was observed for low-density lipoprotein cholesterol (LDL-C). Furthermore, the cDNAs encoding lipoprotein lipase (LPL) and hepatic lipase (HL) were isolated in Amur sturgeon, respectively. The full-length LPL cDNA was composed of 1757 bp with an open reading frame of 501 amino acids, while the complete nucleotide sequences of HL covered 1747 bp encoding 499 amino acids. In the liver, the activities and mRNA levels of LPL were markedly lower in HSD group, which were consistent with the variation tendency of HL. Fish reared in HSD group also presented lower levels of activities and mRNA expression of LPL in the muscle and gonad. Moreover, the expressions of peroxisome proliferator-activated receptor α (PPARα) in both the liver and skeletal muscle were significantly upregulated in HSD group. Overall, the results indicated that high stocking density negatively affects growth performance and lipid deposition of Amur sturgeon to a certain extent. The downregulation of LPL and HL and the upregulation of PPARα may be responsible for the lower lipid distribution of Amur sturgeon in higher stocking density.

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Acknowledgments

This research was funded by the Chinese Special Fund for Agro-scientific Research in the Public Interest (201003055). The authors also thank the Xunlong fisheries farm for providing the Amur sturgeons for the experiments.

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  1. Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, People’s Republic of China

    Yuanyuan Ren, Haishen Wen, Yun Li, Jifang Li, Feng He & Meng Ni

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  1. Yuanyuan Ren
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  2. Haishen Wen
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  3. Yun Li
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Correspondence to Haishen Wen.

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Ren, Y., Wen, H., Li, Y. et al. Effects of stocking density on lipid deposition and expression of lipid-related genes in Amur sturgeon (Acipenser schrenckii). Fish Physiol Biochem 43, 1707–1720 (2017). https://doi.org/10.1007/s10695-017-0403-y

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