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Serum biochemical and non-specific immune responses of rainbow trout (Oncorhynchus mykiss) to dietary nucleotide and chronic stress

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Abstract

The aim of the present study was to investigate whether supplementary nucleotide “Optimun” mitigates the adverse effects of chronic overcrowding in Oncorhynchus mykiss. Two experimental diets [control and nucleotide-supplemented (0.2 %)] and two rearing densities (10 and 30 kg m−3) were combined to have four experimental treatments. The fish were reared for 45 days under different densities using different diets. At the end of the trial, FCR of the fish in higher density was significantly higher than those of the lower density. Nucleotide had no significant effects on growth performance and survival rate. Supplemented nucleotide significantly increased blood hematocrit, whereas it decreased serum total protein, total immunoglobulin (Ig) and creatinine. Overcrowding significantly increased serum glucose and total protein level and decreased serum lysozyme activity, but supplemented nucleotide produced no improvement in these items. No significant effect of overcrowding and dietary nucleotide was observed on serum cortisol. Supplemented nucleotide significantly increased serum urea under low stocking density. Overall, the results showed that 0.2 % “Optimun” had no positive effects on rainbow trout and also caused some immunological and metabolic problems. These findings are not in accordance with those obtained in the same species, with same nucleotide source and level, but acute stress; thus, further studies are encouraged on this topic.

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Authors and Affiliations

  1. Department of Veterinary Medicine, Agrotechnical Institute, Peoples’ Friendship University of Russia, Moscow, Russia

    Morteza Yousefi

  2. Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran

    Mehdi Paktinat

  3. Department of Fisheries, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran

    Nemat Mahmoudi

  4. Departament of Zoology, University of Granada, Campus de Fuentenueva, 18071, Granada, Spain

    Amalia Pérez-Jiménez

  5. Department of Biochemistry and Molecular Biology I, Faculty of Science, University of Granada, 18071, Granada, Spain

    Amalia Pérez-Jiménez

  6. Inland Waters Aquatic Stocks Research Center, Iranian Fisheries Science Research Institute, POB 139, Gorgan, Iran

    Seyyed Morteza Hoseini

Authors
  1. Morteza Yousefi
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  2. Mehdi Paktinat
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  3. Nemat Mahmoudi
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  4. Amalia Pérez-Jiménez
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  5. Seyyed Morteza Hoseini
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Correspondence to Seyyed Morteza Hoseini.

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Yousefi, M., Paktinat, M., Mahmoudi, N. et al. Serum biochemical and non-specific immune responses of rainbow trout (Oncorhynchus mykiss) to dietary nucleotide and chronic stress. Fish Physiol Biochem 42, 1417–1425 (2016). https://doi.org/10.1007/s10695-016-0229-z

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