Skip to main content
SpringerLink
Log in

Dietary lipid levels impact lipoprotein lipase, hormone-sensitive lipase, and fatty acid synthetase gene expression in three tissues of adult GIFT strain of Nile tilapia, Oreochromis niloticus

  • Published:
Fish Physiology and Biochemistry Aims and scope Submit manuscript

Abstract

The objective of this study was to assess the effects of dietary lipids on growth performance, body composition, serum parameters, and expression of genes involved in lipid metabolism in adult genetically improved farmed tilapia (GIFT strain) of Nile tilapia, Oreochromis niloticus. We randomly assigned adult male Nile tilapia (average initial body weight = 220.00 ± 9.54 g) into six groups consisting of four replicates (20 fish per replicate). Fish in each group were hand-fed a semi-purified diets containing different lipid levels [3.3 (the control group), 28.4, 51.4, 75.4, 101.9, and 124.1 g kg−1] for 8 weeks. The results indicated that there was no obvious effect in feeding rate among all groups (P > 0.05). The highest weight gain, specific growth rate, and protein efficiency ratio in 75.4 g kg−1 diet group were increased by 23.31, 16.17, and 22.02 % than that of fish in the control group (P < 0.05). Protein retention ratio was highest in 51.4 g kg−1 diet group. The results revealed that the optimum dietary lipid level for maximum growth performance is 76.6–87.9 g kg−1. Increasing dietary lipid levels contributed to increased tissue and whole body lipid levels. Saturated and monounsaturated fatty acids (MUFAs) decreased, and polyunsaturated fatty acids increased with increasing dietary lipid levels. With the exception of MUFAs, the fatty acid profiles of liver and muscle were similar. Dietary lipid levels were negatively correlated with low-density lipoprotein- cholesterol content and positively with triacylglycerol and glucose contents. In the lipid-fed groups, there was a significant down-regulation of fatty acid synthase (FAS) mRNA in liver, muscle, and visceral adipose tissues. There was a rapid up-regulation of lipoprotein lipase (LPL) mRNA in muscle and liver with increasing dietary lipid levels. In visceral adipose tissue, LPL mRNA was significantly down-regulated in the lipid-fed groups. Dietary lipids increased hormone-sensitive lipase (HSL) mRNA expression levels in the three tissues. These results strongly suggested that moderate dietary lipid levels were beneficial for adult tilapia growth performance and feed efficiency. However, excessive dietary lipid levels contributed to lipid deposition. Additionally, excessive dietary lipids may induce a competition between lipolysis and lipogenesis. FAS did not have tissue-specific regulation; however, the regulation of dietary lipids on LPL expression is tissue specific. FAS was a negative feedback regulator on fat deposition, and HSL was an indicator of fat content in tilapia.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Abdel-Tawwab M (2012) Interactive effects of dietary protein and live bakery yeast, Saccharomyces cerevisiae on growth performance of Nile tilapia, Oreochromis niloticus (L.) fry and their challenge against Aeromonas hydrophila infection. Aquac Int 20:317–331

    Article  CAS  Google Scholar 

  • Ai QH, Zhao JZ, Mai KS, Xu W, Tan BP, Ma HM, Liufu ZG (2008) Optimal dietary lipid level for large yellow croaker (Pseudosciaena crocea) larvae. Aquac Nutr 14:515–522

    Article  CAS  Google Scholar 

  • Albalat A, Saera-Vila A, Capilla E, Gutiérrez J, Pérez-Sánchez J, Navarro I (2007) Insulin regulation of lipoprotein lipase (LPL) activity and expression in gilthead sea bream (Sparus aurata). Comp Biochem Physiol B Biochem Mol Biol 148:151–159

    Article  CAS  PubMed  Google Scholar 

  • Ali M, Jauncey K (2004) Optimal dietary carbohydrate to lipid ratio in African catfish Clarias gariepinus (Burchell 1822). Aquac Int 12:169–180

    Article  CAS  Google Scholar 

  • AOAC (1995) Official methods of analysis, 16th edn. AOAC International, Washington, DC

    Google Scholar 

  • Benedito-Palos L, Calduch-Giner JA, Ballester-Lozano GF, Pérez-Sánchez J (2013) Effect of ration size on fillet fatty acid composition, phospholipid allostasis and mRNA expression patterns of lipid regulatory genes in gilthead sea bream (Sparus aurata). Br J Nutr 109:1175–1187

    Article  CAS  PubMed  Google Scholar 

  • Borges P, Oliveira B, Casal S, Dias J, Conceição L, Valente LM (2009) Dietary lipid level affects growth performance and nutrient utilisation of Senegalese sole (Solea senegalensis) juveniles. Br J Nutr 102:1007–1014

    Article  CAS  PubMed  Google Scholar 

  • Chatzifotis S, Panagiotidou M, Papaioannou N, Pavlidis M, Nengas I, Mylonas CC (2010) Effect of dietary lipid levels on growth, feed utilization, body composition and serum metabolites of meagre (Argyrosomus regius) juveniles. Aquaculture 307:65–70

    Article  CAS  Google Scholar 

  • Chen J, Zhu X, Han D, Yang Y, Lei W, Xie S (2011) Effect of dietary n-3 HUFA on growth performance and tissue fatty acid composition of gibel carp Carassius auratus gibelio. Aquac Nutr 17:e476–e485

    Article  Google Scholar 

  • Chen Q-L, Luo Z, Song Y-F, Wu K, Huang C, Pan Y-X, Zhu Q-L (2014) Hormone-sensitive lipase in yellow catfish Pelteobagrus fulvidraco: molecular characterization, mRNA tissue expression and transcriptional regulation by leptin in vivo and in vitro. Gen Comp Endocrinol 206:130–138

    Article  CAS  PubMed  Google Scholar 

  • Cheng H-L, Meng X-P, Sun S-P, Shi X-Y, Peng Y-X, Dong Z-G, Shen X (2009a) Cloning and expression analysis of a cDNA encoding lipoprotein lipase from the liver of adult grass carp (Ctenopharyngodon idella). Aquac Res 40:1838–1848

    Article  CAS  Google Scholar 

  • Cheng H-L, Wang X, Peng Y-X, Meng X-P, Sun S-P, Shi X-Y (2009b) Molecular cloning and tissue distribution of lipoprotein lipase full-length cDNA from Pengze crucian carp (Carassius auratus var. Pengze). Comp Biochem Physiol B Biochem Mol Biol 153:109–115

    Article  PubMed  Google Scholar 

  • Chou B-S, Shiau S-Y (1996) Optimal dietary lipid level for growth of juvenile hybrid tilapia, Oreochromis niloticus × Oreochromis aureus. Aquaculture 143:185–195

    Article  CAS  Google Scholar 

  • Ding L, Zhang L, Wang J, Ma J, Meng X, Duan P, Sun L, Sun Y (2010) Effect of dietary lipid level on the growth performance, feed utilization, body composition and blood chemistry of juvenile starry flounder (Platichthys stellatus). Aquac Res 41:1470–1478

    CAS  Google Scholar 

  • Du ZY, Liu YJ, Tian LX, Wang JT, Wang Y, Liang GY (2005) Effect of dietary lipid level on growth, feed utilization and body composition by juvenile grass carp (Ctenopharyngodon idella). Aquac Nutr 11:139–146

    Article  CAS  Google Scholar 

  • FAO (2012) The state of world fisheries and aquaculture. Aquaculture Department, pp 56–58

  • Figueiredo-Silva A Cláudia, Panserat Stéphane, Kaushik Sadasivam, Geurden Inge, Polakof Sergio (2012) High levels of dietary fat impair glucose homeostasis in rainbow trout. J Exp Biol 215:169–178

    Article  CAS  PubMed  Google Scholar 

  • Holm C, Østerlund T, Laurell H, Contreras JA (2000) Molecular mechanisms regulating hormone-sensitive lipase and lipolysis. Annu Rev Nutr 20:365–393

    Article  CAS  PubMed  Google Scholar 

  • Hsieh S-L, Hu C-Y, Hsu Y-T, Hsieh T-J (2007) Influence of dietary lipids on the fatty acid composition and stearoyl-CoA desaturase expression in hybrid tilapia (Oreochromis niloticus × O. aureus) under cold shock. Comp Biochem Physiol B Biochem Mol Biol 147:438–444

    Article  PubMed  Google Scholar 

  • Huang C-H, Huang M-C, Hou P-C (1998) Effect of dietary lipids on fatty acid composition and lipid peroxidation in sarcoplasmic reticulum of hybrid tilapia, Oreochromis niloticus × O. aureus. Comp Biochem Physiol B Biochem Mol Biol 120:331–336

    Article  Google Scholar 

  • Huang Y, Liang X-F, Wang L, Li G-Z, Liu X-X, Yao Y (2010) Molecular characterization and evolutional analysis of liportein lipase (LPL) and hepatic lipase (HL) gene in Chinese sturgeon and other six freshwater fishes. Zool Res 31:239–249

    PubMed  Google Scholar 

  • Huang F, Wen H, Wu F, Jiang M, Liu W, Tian J, Shao H (2013) The dietary niacin requirement of large GIFT tilapia. J South China Agric Univ 34:235–240

    CAS  Google Scholar 

  • Jauncey K (2000) Nutritional requirements. In: Beveridge MM, McAndrew B (eds) Tilapias: biology and exploitation, vol 25. Springer, Dordrecht, pp 327–375

    Chapter  Google Scholar 

  • Ji H, Li J, Liu P (2011) Regulation of growth performance and lipid metabolism by dietary n-3 highly unsaturated fatty acids in juvenile grass carp, Ctenopharyngodon idellus. Comp Biochem Physiol B Biochem Mol Biol 159:49–56

    Article  PubMed  Google Scholar 

  • Jiang M, Yao Y, Wen H, Wu F, Liu W, Tian J, Yang C (2013) Dietary available phosphorus requirement of adult GIFT strain of Oreochromis niloticus reared in freshwater. J Fish China 37:1725–1732

    Google Scholar 

  • Kanazawa A, Teshima S, Sakamoto M, Awal MA (1980) Requirements of Tilapia zillii for essential fatty acids. Bull Jpn Soc Sci Fish 46:1353–1356

    Article  CAS  Google Scholar 

  • Kaneko G, Yamada T, Han Y, Hirano Y, Khieokhajonkhet A, Shirakami H, Nagasaka R, Kondo H, Hirono I, Ushio H, Watabe S (2013) Differences in lipid distribution and expression of peroxisome proliferator-activated receptor gamma and lipoprotein lipase genes in torafugu and red seabream. Gen Comp Endocrinol 184:51–60

    Article  CAS  PubMed  Google Scholar 

  • Katan MB, Zock PL, Mensink RP (1995) Dietary oils, serum lipoproteins, and coronary heart disease. Am J Clin Nutr 61:1368S–1373S

    CAS  PubMed  Google Scholar 

  • Kazala EC, Petrak JL, Lozeman FJ, Mir PS, Laroche A, Deng J, Weselake RJ (2003) Hormone-sensitive lipase activity in relation to fat content of muscle in Wagyu hybrid cattle. Livest Prod Sci 79:87–96

    Article  Google Scholar 

  • Kennedy SR, Bickerdike R, Berge RK, Porter AR, Tocher DR (2007) Influence of dietary conjugated linoleic acid (CLA) and tetradecylthioacetic acid (TTA) on growth, lipid composition and key enzymes of fatty acid oxidation in liver and muscle of Atlantic cod (Gadus morhua L.). Aquaculture 264:372–382

    Article  CAS  Google Scholar 

  • Kikuchi K, Furuta T, Iwata N, Onuki K, Noguchi T (2009) Effect of dietary lipid levels on the growth, feed utilization, body composition and blood characteristics of tiger puffer Takifugu rubripes. Aquaculture 298:111–117

    Article  CAS  Google Scholar 

  • Kittilson JD, Reindl KM, Sheridan MA (2011) Rainbow trout (Oncorhynchus mykiss) possess two hormone-sensitive lipase-encoding mRNAs that are differentially expressed and independently regulated by nutritional state. Comp Biochem Physiol A Mol Integr Physiol 158:52–60

    Article  PubMed  Google Scholar 

  • Kleveland EJ, Ruyter B, Vegusdal A, Sundvold H, Berge RK, Gjøen T (2006) Effects of 3-thia fatty acids on expression of some lipid related genes in Atlantic salmon (Salmo salar L.). Comp Biochem Physiol B Biochem Mol Biol 145:239–248

    Article  PubMed  Google Scholar 

  • Lampidonis AD, Rogdakis E, Voutsinas GE, Stravopodis DJ (2011) The resurgence of hormone-sensitive lipase (HSL) in mammalian lipolysis. Gene 477:1–11

    Article  CAS  PubMed  Google Scholar 

  • Lee S-M, Lee JH, Kim K-D (2003) Effect of dietary essential fatty acids on growth, body composition and blood chemistry of juvenile starry flounder (Platichthys stellatus). Aquaculture 225:269–281

    Article  CAS  Google Scholar 

  • Leng XJ, Wu XF, Tian J, Li XQ, Guan L, Weng DC (2012) Molecular cloning of fatty acid synthase from grass carp (Ctenopharyngodon idella) and the regulation of its expression by dietary fat level. Aquac Nutr 18:551–558

    Article  CAS  Google Scholar 

  • Li S-F, Tang S-J, Cai W-Q (2010) RAPD-SCAR markers for genetically improved NEW GIFT Nile tilapia (Oreochromis niloticus niloticus L.) and their application in strain identification. Zool Res 31:147–153

    Article  PubMed  Google Scholar 

  • Liang X-F, Ogata HY, Oku H (2002a) Effect of dietary fatty acids on lipoprotein lipase gene expression in the liver and visceral adipose tissue of fed and starved red sea bream Pagrus major. Comp Biochem Physiol A Mol Integr Physiol 132:913–919

    Article  PubMed  Google Scholar 

  • Liang X-F, Oku H, Ogata HY, Li Y-Q, Bai J-J, Luo J-R (2002b) The cDNA sequence and tissue expression of lipoprotein lipase gene of a Marine Fish, Red Sea Bream (Pagrus major). Chin J Biochem Mol Biol 18:712–719

    CAS  Google Scholar 

  • Liang XF, Oku H, Ogata HY (2002c) The effects of feeding condition and dietary lipid level on lipoprotein lipase gene expression in liver and visceral adipose tissue of red sea bream Pagrus major. Comp Biochem Physiol A Mol Integr Physiol 131:335–342

    Article  PubMed  Google Scholar 

  • Lichtenstein AH (2006) Thematic review series: patient-oriented research. Dietary fat, carbohydrate, and protein: effects on plasma lipoprotein patterns. J Lipid Res 47:1661–1667

    Article  CAS  PubMed  Google Scholar 

  • Lim C, Yildirim-Aksoy M, Klesius P (2011) Lipid and fatty acid requirements of tilapias. N Am J Aquac 73:188–193

    Article  Google Scholar 

  • Liu Z, Yang F, Kong L, Lai C, Piao X, Gu Y, Ou X (2007) Effects of dietary energy density on growth, carcass quality and mRNA expression of fatty acid synthase and hormone-sensitive lipase in finishing pigs. Asian-Australasian J Anim Sci 20:1587–1593

    Article  CAS  Google Scholar 

  • Liu X, Liu H, Chen Y, Du G, Chen J (2008) Effects of organic matter and initial carbon–nitrogen ratio on the bioconversion of volatile fatty acids from sewage sludge. J Chem Technol Biotechnol 83:1049–1055

    Article  CAS  Google Scholar 

  • López LM, Durazo E, Viana MT, Drawbridge M, Bureau DP (2009) Effect of dietary lipid levels on performance, body composition and fatty acid profile of juvenile white seabass, Atractoscion nobilis. Aquaculture 289:101–105

    Article  Google Scholar 

  • Luo G, Xu J, Teng Y, Ding C, Yan B (2010) Effects of dietary lipid levels on the growth, digestive enzyme, feed utilization and fatty acid composition of Japanese sea bass (Lateolabrax japonicus L.) reared in freshwater. Aquac Res 41:210–219

    Article  CAS  Google Scholar 

  • Makol A, Torrecillas S, Caballero MJ, Fernández-Vaquero A, Izquierdo MS (2012) Effect of long term feeding with conjugated linoleic acid (CLA) in growth performance and lipid metabolism of European sea bass (Dicentrarchus labrax). Aquaculture 368–369:129–137

    Article  Google Scholar 

  • Martinez-Rubio L, Wadsworth S, González Vecino JL, Bell JG, Tocher DR (2013) Effect of dietary digestible energy content on expression of genes of lipid metabolism and LC-PUFA biosynthesis in liver of Atlantic salmon (Salmo salar L.). Aquaculture 384–387:94–103

    Article  Google Scholar 

  • Martins DA, Valente LMP, Lall SP (2007) Effects of dietary lipid level on growth and lipid utilization by juvenile Atlantic halibut (Hippoglossus hippoglossus, L.). Aquaculture 263:150–158

    Article  CAS  Google Scholar 

  • Masuno H, Tsujita T, Nakanishi H, Yoshida A, Fukunishi R, Okuda H (1984) Lipoprotein lipase-like activity in the liver of mice with Sarcoma 180. J Lipid Res 25:419–427

    CAS  PubMed  Google Scholar 

  • Mensink RP, Katan MB (1992) Effect of dietary fatty acids on serum lipids and lipoproteins. A meta-analysis of 27 trials. Arterioscler Thromb Vasc Biol 12:911–919

    Article  CAS  Google Scholar 

  • Mensink RP, Zock PL, Kester AD, Katan MB (2003) Effects of dietary fatty acids and carbohydrates on the ratio of serum total to HDL cholesterol and on serum lipids and apolipoproteins: a meta-analysis of 60 controlled trials. Am J Clin Nutr 77:1146–1155

    CAS  PubMed  Google Scholar 

  • Merkel M, Weinstock PH, Chajek-Shaul T, Radner H, Yin B, Breslow JL, Goldberg IJ (1998) Lipoprotein lipase expression exclusively in liver. A mouse model for metabolism in the neonatal period and during cachexia. J Clin Invest 102:893

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Mjoun K, Rosentrater KA, Brown ML (2010) Tilapia: environmental biology and nutritional requirements

  • Morais S, Castanheira F, Martinez-Rubio L, Conceição LEC, Tocher DR (2012) Long chain polyunsaturated fatty acid synthesis in a marine vertebrate: ontogenetic and nutritional regulation of a fatty acyl desaturase with Δ4 activity. Biochim Biophys Acta Mole Cell Biol of Lipids 1821:660–671

    Article  CAS  Google Scholar 

  • Peres H, Oliva-Teles A (1999) Effect of dietary lipid level on growth performance and feed utilization by European sea bass juveniles (Dicentrarchus labrax). Aquaculture 179:325–334

    Article  CAS  Google Scholar 

  • Polakof S, Médale F, Larroquet L, Vachot C, Corraze G, Panserat S (2011) Insulin stimulates lipogenesis and attenuates beta-oxidation in white adipose tissue of fed rainbow trout. Lipids 46:189–199

    Article  CAS  PubMed  Google Scholar 

  • Preiss-Landl K, Zimmermann R, Hämmerle G, Zechner R (2002) Lipoprotein lipase: the regulation of tissue specific expression and its role in lipid and energy metabolism. Curr Opin Lipidol 13:471–481

    Article  CAS  PubMed  Google Scholar 

  • Regost C, Arzel J, Cardinal M, Robin J, Laroche M, Kaushik S (2001) Dietary lipid level, hepatic lipogenesis and flesh quality in turbot (Psetta maxima). Aquaculture 193:291–309

    Article  CAS  Google Scholar 

  • Santiago CB, Reyes OS (1993) Effects of dietary lipid source on reproductive performance and tissue lipid levels of Nile tilapia Oreochromis niloticus (Linnaeus) broodstock. J Appl Ichthyol 9:33–40

    Article  CAS  Google Scholar 

  • Semenkovich CF (1997) Regulation of fatty acid synthase (FAS). Prog Lipid Res 36:43–53

    Article  CAS  PubMed  Google Scholar 

  • Shao H, Wen H, Liu W, Jiang M, Wu F, Tian J, Huang F (2013) Dietary choline requirements of adult GIFT tilapia (Oreochromis niloticus). J Fish Sci China 20:1007–1014

    CAS  Google Scholar 

  • Takeuchi T, Satoh S, Watanabe T (1983) Requirement of Tilapia nilotica for essential fatty acids. Bull Jpn Soc Sci Fish 49

  • Tanomman S, Ketudat-Cairns M, Jangprai A, Boonanuntanasarn S (2013) Characterization of fatty acid delta-6 desaturase gene in Nile tilapia and heterogenous expression in Saccharomyces cerevisiae. Comp Biochem Physiol B Biochem Mol Biol 166:148–156

    Article  CAS  PubMed  Google Scholar 

  • Teoh C-Y, Turchini GM, Ng W-K (2011) Erratum to “Genetically improved farmed Nile tilapia and red hybrid tilapia showed differences in fatty acid metabolism when fed diets with added fish oil or a vegetable oil blend”. Aquaculture 316:144–154

    Article  Google Scholar 

  • Tian J, Wen H, Zeng L-B, Jiang M, Wu F, Liu W, Yang C-G (2013) Changes in the activities and mRNA expression levels of lipoprotein lipase (LPL), hormone-sensitive lipase (HSL) and fatty acid synthetase (FAS) of Nile tilapia (Oreochromis niloticus) during fasting and re-feeding. Aquaculture 400–401:29–35

    Article  Google Scholar 

  • Tocher DR (2010) Fatty acid requirements in ontogeny of marine and freshwater fish. Aquac Res 41:717–732

    Article  CAS  Google Scholar 

  • Tocher DR, Zheng X, Schlechtriem C, Hastings N et al (2006) Highly unsaturated fatty acid synthesis in marine fish: cloning, functional characterization, and nutritional regulation of fatty acyl [delta]6 desaturase of Atlantic cod (Gadus morhua L.). Lipids 41:1003–1016

    Article  CAS  PubMed  Google Scholar 

  • Tu W, Tian J, Wen H, Jiang M, Wu F, Liu W, Zhang M, Sun L (2012) Optimal dietary lipid requirement of advanced juvenile Nile tilapia Oreochromis niloticus. J Fish Sci China 19:436–444

    CAS  Google Scholar 

  • Turchini GM, Torstensen BE, Ng W-K (2009) Fish oil replacement in finfish nutrition. Rev Aquac 1:10–57

    Article  Google Scholar 

  • Wakil SJ (1989) Fatty acid synthase, a proficient multifunctional enzyme. Biochemistry 28:4523–4530

    Article  CAS  PubMed  Google Scholar 

  • Wang H, Eckel RH (2009) Lipoprotein lipase: from gene to obesity. Am J Physiol Endocrinol Metabol 297:E271–E288

    Article  CAS  Google Scholar 

  • Wang J-T, Liu Y-J, Tian L-X, Mai K-S, Du Z-Y, Wang Y, Yang H-J (2005) Effect of dietary lipid level on growth performance, lipid deposition, hepatic lipogenesis in juvenile cobia (Rachycentron canadum). Aquaculture 249:439–447

    Article  CAS  Google Scholar 

  • Wang A, Han G, Wei X, Liu B, Lv F, Feng G, Qi Z, Wang T, Xu P, Yang Z (2010) Molecular cloning of fatty acid synthase from GIFT tilapia (Oreochromis niloticus): response of its expression to refeeding and different lipid level in diet. J Fish Sci China 34:1113–1120

    CAS  Google Scholar 

  • Wang A, Han G, Qi Z, Lv F, Yu Y, Huang J, Wang T, Xu P (2013) Cloning of lipoprotein lipase (LPL) and the effects of dietary lipid levels on LPL expression in GIFT tilapia (Oreochromis niloticus). Aquac Int 1–14

  • Weil C, Lefèvre F, Bugeon J (2013) Characteristics and metabolism of different adipose tissues in fish. Rev Fish Biol Fish 23:157–173

    Article  Google Scholar 

  • Wu F, Wen H, Jiang M, Liu W, Liu B, Tian J (2011) Effects of different dietary carbohydrate levels on growth performance, body composition and serum biochemical indices of juvenile hybrid tilapia (Oreochromis niloticus♀ × O. aureus♂). J South China Agric Univ 32:91–95

    Google Scholar 

  • Wu F, Wen H, Jiang M, Liu W, Liu B, Tian J, Yang C (2012) Effects of different dietary carbohydrate levels on growth performance and blood biochemical parameters of juvenile GIFT tilapia (Oreochromis niloticus). J Northwest Agric For Univ 40:8–14

    Google Scholar 

  • Xie D, Chen F, Lin S, Wang S, You C, Monroig Ó, Tocher DR, Li Y (2014) Cloning, functional characterization and nutritional regulation of Δ6 fatty acyl desaturase in the herbivorous euryhaline teleost Scatophagus Argus. PLoS ONE 9:e90200

    Article  PubMed Central  PubMed  Google Scholar 

  • Yao YF, Jiang M, Wen H, Wu F, Liu W, Tian J, Yang CG (2014) Dietary phosphorus requirement of GIFT strain of Nile tilapia Oreochromis niloticus reared in freshwater. Aquac Nutr 20:273–281

    Article  CAS  Google Scholar 

  • Zamora-Sillero J, Ramos LRV, Romano LA, Monserrat JM, Tesser MB (2013) Effect of dietary dextrin levels on the growth performance, blood chemistry, body composition, hepatic triglicerides and glycogen of Lebranche mullet juveniles (Mugil liza Valenciennes 1836, Mugilidae). J Appl Ichthyol 29:1342–1347

    Article  CAS  Google Scholar 

  • Zheng X, Ding Z, Xu Y, Monroig O, Morais S, Tocher DR (2009) Physiological roles of fatty acyl desaturases and elongases in marine fish: characterisation of cDNAs of fatty acyl Δ6 desaturase and elovl5 elongase of cobia (Rachycentron canadum). Aquaculture 290:122–131

    Article  CAS  Google Scholar 

  • Zheng K, Zhu X, Han D, Yang Y, Lei W, Xie S (2010) Effects of dietary lipid levels on growth, survival and lipid metabolism during early ontogeny of Pelteobagrus vachelli larvae. Aquaculture 299:121–127

    Article  CAS  Google Scholar 

  • Zuo R, Ai Q, Mai K, Xu W, Wang J, Xu H, Liufu Z, Zhang Y (2012) Effects of dietary n-3 highly unsaturated fatty acids on growth, nonspecific immunity, expression of some immune related genes and disease resistance of large yellow croaker (Larmichthys crocea) following natural infestation of parasites (Cryptocaryon irritans). Fish Shellfish Immunol 32:249–258

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We thank Ling-Bing Zeng, Zhong Li, and Gui-Wei Zou for providing essential facilities during the qRT-PCR analysis. This work was supported by Grants from the Special Fund for Agro-scientific Research in the Public Interest (No. 201003020) and the China Agriculture Research System (No. CARS-49).

Author information

Authors and Affiliations

  1. Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 8, Wudayuan 1st Road, Donghu Hi-Tech Development Zone, Wuhan, 430223, Hubei, China

    Juan Tian, Fan Wu, Chang-Geng Yang, Ming Jiang, Wei Liu & Hua Wen

  2. Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan, 430070, China

    Juan Tian & Ming Jiang

Authors
  1. Juan Tian
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fan Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chang-Geng Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ming Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Wei Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hua Wen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hua Wen.

Additional information

Juan Tian and Fan Wu have contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tian, J., Wu, F., Yang, CG. et al. Dietary lipid levels impact lipoprotein lipase, hormone-sensitive lipase, and fatty acid synthetase gene expression in three tissues of adult GIFT strain of Nile tilapia, Oreochromis niloticus . Fish Physiol Biochem 41, 1–18 (2015). https://doi.org/10.1007/s10695-014-0001-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10695-014-0001-1

Keywords

Search

Navigation