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Association of monoallelic MUTYH mutation among Egyptian patients with colorectal cancer

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Abstract

Colorectal cancer (CRC) is a heterogeneous triat that involves both environmental and genetic factors. Genetic mutations of MUTYH (p.Y179C and p.G396D) have been reported to be associated with increased risk of CRC among several ethnic populations. The aim of this work is to assess the association of the monoallelic MUTYH mutations (p.Y179C and p.G396D) with increased risk of CRC among Egyptian patients. This study included 120 unrelated CRC Egyptian patients who were compared with 100 healthy controls from the same locality. For all individuals, DNA was genotyped for MUTYH p.Y179C and MUTYH p.G396D mutations using the T-ARMS-PCR technique. The frequencies of monoallelic MUTYH mutations showed a strong association with the increased risk of CRC among Egyptian patients compared with controls (12.5 vs. 4.0 %, OR = 3.49, 95 % CI = 1.12–10.90, P = 0.03). Moreover, the frequency of MUTYH p.Y179C mutation was noted to be significantly higher among CRC patients compared to controls rather than MUTYH p.G396D mutation. Interestingly, CRC patients with tumors in the right side colon showed an evidence for association with the MUTYH p.Y179C mutation compared with tumors in the left side colon (p = 0.01). MUTYH p.Y179C mutation was associated with an increased risk of CRC among Egyptian patients rather than MUTYH p.G396D mutation.

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Acknowledgments

Authors are grateful for the staff members of Damietta Cancer Institute, Damietta, Egypt for their help in selecting cases under the study.

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    Authors and Affiliations

    1. Genetics Unit, Children Hospital, Mansoura University, Mansoura, Egypt

      Afaf Elsaid

    2. Department of Biochemistry, College of Science, Tanta University, Tanta, Egypt

      Rami Elshazli

    3. Department of Biochemistry, College of Science, Damietta University, Damietta, Egypt

      Fatma El-Tarapely & Camelia Abdel-Malak

    4. Medical Oncology Department, Damietta Cancer Institute, Damietta, Egypt

      Hossam Darwish

    5. Clinical Chemistry Lab, Talkha Central Hospital (TCH), Mansoura, Egypt

      Rami Elshazli

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    1. Afaf Elsaid
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    Dr. Rami Elshazli and Dr. Afaf Elsaid have contributed equally to this work.

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    Elsaid, A., Elshazli, R., El-Tarapely, F. et al. Association of monoallelic MUTYH mutation among Egyptian patients with colorectal cancer. Familial Cancer 16, 83–90 (2017). https://doi.org/10.1007/s10689-016-9927-z

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