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Identification of natural sources of resistance to bipartite begomovirus TYLCKaV in Solanum melongena

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Abstract

Yellow leaf curl disease caused by begomoviruses has emerged as a major limitation to the production of eggplant (Solanum melongena) in several regions of the world. Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV) is a bipartite begomovirus isolated from diseased eggplant in the areas of the Indochinese peninsula, South China, and Maritime Southeast Asia. In this study, five begomovirus species isolated in Indonesia, including TYLCKaV, were inoculated to eggplant using infectious clones. Among the inoculated begomoviruses, TYLCKaV alone induced typical yellowing and curling symptoms in eggplant. Inoculation of TYLCKaV to a panel of crop species (eggplant, tomato, pepper, tobacco, cucumber, melon, and squash) commonly grown in Indonesia clarified that TYLCKaV infects and causes yellow leaf curl disease mainly in eggplant and tomato. Agroinfiltration of TYLCKaV to 736 accessions of eggplant enabled selection of 33 resistant candidates. Further evaluation by TYLCKaV graft-inoculation of the resistant candidates clarified that 10 accessions were classifiable as moderately resistant and 23 accessions as highly resistant. Among the highly resistant accessions, 22 accessions restricted viral DNA accumulation to a significantly lower amount than the susceptible control. These 22 highly resistant accessions represent valuable genetic resources for breeding begomovirus resistance in eggplant.

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Acknowledgements

We thank Shinya Kanzaki (Kindai University) for useful discussion. We thank Robert McKenzie, PhD, from Edanz (https://jp.edanz.com/ac), for editing a draft of this manuscript. Most of the plant materials were kindly provided by the NARO Genebank. We thank the constructive comments and suggestion made by the anonymous reviewers which helped to improve the manuscript.

Funding

This work was supported by JSPS KAKENHI Grant Number 19H02950, 21KK0109, and 23H02207 for S. Koeda, and JSPS-DG-RSTHE Joint Research Program for S. Koeda and E. Kesumawati.

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Authors and Affiliations

  1. Graduate School of Agriculture, Kindai University, Nara, Nara, 631-8505, Japan

    Kyohei Kikkawa & Sota Koeda

  2. Faculty of Agriculture, Kindai University, Nara, Nara, 631-8505, Japan

    Minami Tanaka & Sota Koeda

  3. Faculty of Agriculture, Syiah Kuala University, Darussalam, Banda Aceh, 23111, Indonesia

    Elly Kesumawati

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  1. Kyohei Kikkawa
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  2. Minami Tanaka
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  3. Elly Kesumawati
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  4. Sota Koeda
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Contributions

KK and SK designed experiment, interpreted the results, and wrote the manuscript. KK analyzed the host range of TYLCKaV and evaluated the begomovirus resistance of eggplants. MT prepared the infectious clone of AYVV. EK provided the material. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Sota Koeda.

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Kikkawa, K., Tanaka, M., Kesumawati, E. et al. Identification of natural sources of resistance to bipartite begomovirus TYLCKaV in Solanum melongena. Euphytica 219, 51 (2023). https://doi.org/10.1007/s10681-023-03180-7

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