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Near-isogenic lines of soybean confirm a QTL for seed waterlogging tolerance at different temperatures

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Abstract

Climate change is predicted to increase the probability of soil waterlogging due to severe rainfall, causing significant damage to soybean at the germination stage. Germination under waterlogging is also greatly influenced by temperature. To clarify the variation in germination responses of soybean genotypes to waterlogging at different temperatures, the seeds of 15 soybean genotypes were treated by soaking for 2 days at four temperatures: 21 °C, 23 °C, 25 °C, 27 °C and 29 °C. Differences in the germination rate (GR) and normal seedling rate (NSR) were observed among soybean genotypes after soaking treatments regardless of the temperature. Among the examined genotypes, Iyodaizu was classified as waterlogging tolerant at the germination stage, and Tachinagaha was classified as sensitive. Interestingly, through the analyses of recombinant inbred lines (RILs) developed from a cross between Tachinagaha and Iyodaizu, quantitative trait loci (QTLs) for root development under hypoxia at the seedling stage of soybean were detected on chromosome 12 (Chr.12).We investigated whether the candidate QTL region for root development is involved in seed waterlogging tolerance by using a near-isogenic line (NIL), NIL-9-4-5. Interestingly, under soaking treatment, the GR and NSR of NIL-9-4-5, carrying the candidate QTL region, was nearly the same as that of Iyodaizu and was significantly higher than that of Tachinagaha. These results may indicate that the candidate QTL region for root development under hypoxia at the seedling stage located on Chr.12 contributes to the seed waterlogging tolerance of soybean plants at the germination stage.

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Abbreviations

Chr.:

Chromosome

GR:

Germination rate

NIL:

Near isogenic line

NSR:

Normal seedling rate

QTL:

Quantitative trait loci

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Acknowledgements

This work was supported by grants from key project of Vietnam National University of Agriculture (T2019-01-01TĐ) and TIFO postdoctoral Fellowship.

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Authors and Affiliations

  1. Faculty of Agronomy, Vietnam National University of Agriculture, Trau Quy, Gia Lam, Hanoi, 131000, Vietnam

    Van Loc Nguyen

  2. Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 744, Motooka, Nishi-ku, Fukuoka, 819-0395, Japan

    Thi Thu Hien Dang

  3. Agricultural Genetics Institute, Pham Van Dong, Tu Liem, Hanoi, 131000, Vietnam

    Ha Duc Chu

  4. Faculty of Agriculture, Kyushu University, 111 Harumachi, Kasuya-cho, Kasuya-gun, Fukuoka, 811-2307, Japan

    Tetsuhiro Nakamura, Tomomi Abiko & Toshihiro Mochizuki

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  1. Van Loc Nguyen
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Contributions

LVN designed the methodology. TM and TA developed experimental materials. TM, TA and TN oversaw and guided experiments, data analysis, writing and editing. LVN, HDTT, and HDC conducted the experiments and analysed the data. LVN and HDC wrote the manuscript. All authors read and approved the final manuscript.

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Correspondence to Van Loc Nguyen.

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Nguyen, V.L., Dang, T.T.H., Chu, H.D. et al. Near-isogenic lines of soybean confirm a QTL for seed waterlogging tolerance at different temperatures. Euphytica 217, 16 (2021). https://doi.org/10.1007/s10681-020-02736-1

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