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Genetic mapping and molecular marker development for the gene Pre2 controlling purple grains in barley

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Abstract

Purple grains in barley are caused by the presence of anthocyanin in both aleurone and pericarp, and the trait is controlled by one or two dominant genes. In this study, we demonstrated that purple grain was controlled by a single dominant gene using reciprocal crosses between Yuyaohongdamai and AC Parkhill or ACCA. The close linkage between grain color and spike type (controlled by Vrs1) was found in the crosses between Yuyaohongdamai and ACCA. The purple-grain gene was mapped on chromosome 2HL between the SSR markers Bmag0125 (1.2 cM) and GBMS244 (2.8 cM), and was about 5.1 cM distal from Vrs1. The marker enrichment in the target region was carried out by amplifying barley EST- and rice homologous gene-based searching for polymorphic loci adjacent to Pre2. Four of the newly developed markers were mapped in the Pre2 gene region. The purple lemma and pericarp gene (Pre2) was then finely mapped between InDel marker PQJ1056 (from EST BM096525) and HvOs04g47170 with genetic distance of 0.3 and 0.1 cM, respectively.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (Grant No. 31471495), Science and Technology Innovation Team Project of Zhejiang Province (2011R50026-9) and China Agriculture Research System (CARS-05).

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Authors and Affiliations

  1. Institute of Crop Science, Zhejiang University, Hangzhou, 310058, China

    Qiaojun Jia & Guoping Zhang

  2. Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Qiaojun Jia, Jinghuan Zhu, Junmei Wang & Jianming Yang

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  1. Qiaojun Jia
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  2. Jinghuan Zhu
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  3. Junmei Wang
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  4. Jianming Yang
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  5. Guoping Zhang
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Correspondence to Jianming Yang or Guoping Zhang.

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Jia, Q., Zhu, J., Wang, J. et al. Genetic mapping and molecular marker development for the gene Pre2 controlling purple grains in barley. Euphytica 208, 215–223 (2016). https://doi.org/10.1007/s10681-015-1593-y

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