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Screening a variable germplasm collection of Cucumis melo L. for seedling resistance to Macrophomina phaseolina

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Abstract

We evaluate the seedling resistance to charcoal rot caused by Macrophomina phaseolina in ninety-seven Cucumis melo accessions, from different geographical origins and five F1 generations, derived from crosses of five accessions selected for their resistance. Artificial inoculations with the toothpick method, previously reported to be useful for predicting shoot resistance, were performed, and plants were scored using a scale of disease severity. The average disease severity was calculated for each accession and was used to cluster the accession in five reaction classes. The screening revealed that sources of natural resistance to this fungus are limited. However, seedlings of seven accessions of different botanic groups displayed a resistant response to the stem inoculation, one cantaloup from Israel, one conomon accession from Korea, two wild agrestis and one acidulus from Africa, and two dudaim accessions from Middle East. The response of the F1 progenies varied from susceptibility to high resistance, the latter in progenies from the two agrestis wild types. These results suggest differences in the genetic basis of the resistance in the different selected sources. The resistant accessions are suggested to be screened under field conditions to confirm the level of resistance at adult plant stage and under stressful conditions.

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Acknowledgments

This work has been partially funded by the Project No 294/13 of the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior CAPES (Brazil). M. M. Q. Ambrósio and A. C. A. Dantas thank CAPES for their research fellowships. B.Pico thanks the Programa Hispano–Brasileño de Cooperación Universitaria HBP2012-008 and PHBP14/00021 and to the MINECO project AGL2014-53398-C2-2-R.

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Authors and Affiliations

  1. Department of Plant Sciences, Universidade Federal Rural do Semi-Árido (UFERSA), Av. Francisco Mota, 572, Mossoró, CEP 59.625-900, Brazil

    Márcia M. Q. Ambrósio, Ana C. A. Dantas, Alexis C. Medeiros & Glauber H. S. Nunes

  2. Institute for the Conservation and Breeding of Agricultural Biodiversity (COMAV) of the Universitat Politècnica de València (UPV), Camino de Vera, s/n, 46022, València, Spain

    Eva Martínez-Perez & María Belén Picó

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  1. Márcia M. Q. Ambrósio
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  2. Ana C. A. Dantas
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  3. Eva Martínez-Perez
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  4. Alexis C. Medeiros
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  5. Glauber H. S. Nunes
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  6. María Belén Picó
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Correspondence to María Belén Picó.

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Ambrósio, M.M.Q., Dantas, A.C.A., Martínez-Perez, E. et al. Screening a variable germplasm collection of Cucumis melo L. for seedling resistance to Macrophomina phaseolina . Euphytica 206, 287–300 (2015). https://doi.org/10.1007/s10681-015-1452-x

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