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Tissue-dependent somaclonal mutation frequencies and spectra enhanced by ion beam irradiation in chrysanthemum

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Abstract

We investigated the effect of tissue source selection in combination with ion beam irradiation on generating flower-color mutants in Chrysanthemum morifolium. Flower color mutation in the chrysanthemum cultivar YoMystery (purple flowers) was used as an index to estimate mutation frequency and the spectrum of mutations. Somaclones regenerated from petal and leaf tissues and ion-beam irradiated clones derived from the two tissues were analyzed for stem length and flower color mutation. Three Gy of argon ion beams was regarded as an appropriate dose for irradiation because the resulting color mutants maintained an adequate stem length suitable for commercial use even with high mutation frequencies. Statistical analysis of 7,258 individuals from three separate experiments revealed that (1) somaclones from petal tissue had a higher mutation frequency (2.91 %, σ = 0.55) than leaf somaclones (2.01 %, σ = 0.26); (2) the effect of tissue source on the frequency of flower color mutations was significantly enhanced when combined with ion-beam irradiation in that the mutation frequency of ion-beam irradiated petal clones was 6.47 % (σ = 0.77), whereas that of ion-beam irradiated leaf clones was 3.89 % (σ = 0.52); and (3) the spectrum of carotenoid color mutations was remarkably increased by ion-beam irradiation in that both leaf and petal ion-beam irradiated clones had brown, red and yellow flowers in contrast to the somaclones that only produced yellow flowers. These results suggest that intentional or directed induction of flower color mutants with practical value is possible by combining tissue source selection with ion beam irradiation.

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Notes

  1. E: Accelerated beam energy of the particle in MeV/u (MeV/a.m.u. or MeV/nucleon).

  2. LET: Linear Energy Transfer, (Rate of energy dissipation along the path of charged particles. In radiobiology and health physics, exposure is measured in kiloelectron volts per micrometer of tissue (keV/micrometer)).

References

  • Broertjes C, van Harten AM (1988) Applied mutation breeding for vegetatively propagated crops. Elsevier Science Publisher B. V, Amsterdam

    Google Scholar 

  • Bush SR, Earle ED, Langhans RW (1976) Plantlets from petal segments, petal epidermis and shoot tips of the periclinal chimera Chrysanthemum morifolium ‘Indianapolis’. Am J Bot 63:729–737

    Article  Google Scholar 

  • Chen SM, Li CH, Zhu XR, Deng YM, Sun W, Wang LS, Chen FD, Zhangi Z (2012) The identification of flavonoids and the expression of genes of anthocyanin biosynthesis in the chrysanthemum flowers. Biologia Plantarun 56:458–464

    Article  CAS  Google Scholar 

  • De Jong J, Custers JBM (1986) Induced changes in growth and flowering of chrysanthemum after irradiation and in vitro culture of pedicels and petal epidermis. Euphytica 35:137–148

    Article  Google Scholar 

  • Elia MC, Bradley MO (1992) Influence of chromatin structure on the induction of DNA double strand breaks by ionizing radiation. Cancer Res 52:1580–1586

    CAS  PubMed  Google Scholar 

  • Evans DA (1989) Somaclonal variation-genetic basis and breeding applications. Trend Genet 5:346–350

    Article  Google Scholar 

  • Hase Y, Akita Y, Kitamura S, Narumi I, Tanaka A (2012) Development of an efficient mutagenesis technique using ion beams: toward more controlled mutation breeding. Plant Biotechnol 29:193–200. doi:10.5511/plantbiotechnology.12.0106a

    Article  CAS  Google Scholar 

  • Inagaki Y, Hisatomi Y, Suzuki T, Kasahara K, Iida S (1994) Isolation of a suppressor-mutator/enhancer-like transposable element, Tpn1, from Japanese morning glory bearing variegated flowers. Plant Cell 6:375–383

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Itoh Y, Higeta D, Suzuki A, Yoshida H, Ozeki Y (2002) Excision of transposable elements from the chalcone isomerase and dihydroflavonol 4-reductase genes may contribute to the variegation of the yellow-flowered carnation (Dianthus caryophyllus). Plant Cell Physiol 43:578–585. doi:10.1093/pcp/pcf065

    Article  CAS  PubMed  Google Scholar 

  • Jain SM (2001) Tissue culture-derived variation in crop improvement. Euphytica 118:153–166

    Article  CAS  Google Scholar 

  • Karp A (1995) Somaclonal variation as a tool for crop improvement. Euphytica 85:295–302

    Article  Google Scholar 

  • Kishimoto S, Ohmiya A (2006) Regulation of carotenoid biosynthesis in petals and leaves of chrysanthemum (Chrysanthemum morifolium). Physiol Plantarum 128:436–447

    Article  CAS  Google Scholar 

  • Ladygin VG (2000) Biosynthesis of carotenoids in plastids of plants. Biochem Biokhimiia 65:1113–1128

    CAS  Google Scholar 

  • Larkin PJ, Scowcroft WR (1981) Somaclonal variation—a novel source of variability from cell culture for plant improvement. Theor Appl Genet 60:197–214

    Article  CAS  PubMed  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plantarum 15:473–497

    Article  CAS  Google Scholar 

  • Nagatomi S, Tanaka A, Watanabe H, Tano S (1997) Chrysanthemum mutants regenerated from in vitro explants irradiated with 12C5 ion beam. Institute of radiation breeding, technical news No.60

  • Newman HC, Prise KM, Michael BD (2000) The role of higher-order chromatin structure in the yield and distribution of DNA double-strand breaks in cells irradiated with X-rays or alpha-particles. Int J Radiat Biol 76:1085–1093

    Article  CAS  PubMed  Google Scholar 

  • Ohmiya A, Kishimoto S, Aida R, Yoshioka S, Sumitomo K (2006) Carotenoid cleavage deoxygenase (CmCCD4a) contributes to white color formation in chrysanthemum petals. Plant Physiol 142:1193–1201. doi:10.1104/pp.106.087130

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Okamura M (1994) Pomato: potato protoplast system and somatic hybridization between potato and a wild tomato. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, somatic hybridization in crop improvement I. Springer-Verlag, New York, pp 209–223

    Chapter  Google Scholar 

  • Okamura M, Yasuno N, Ohtsuka M, Tanaka A, Shikazono N, Hase Y (2003) Wide variety of flower-color and -shape mutants regenerated from leaf cultures irradiated with ion beams. Nucl Instrum Methods Phys Res B 206:574–578. doi:10.1016/S0168-583X(03)00835-8

    Article  CAS  Google Scholar 

  • Okamura M, Tanaka A, Momose M, Umemoto N, da Silva JAT, Toguri T (2006) Advances of mutagenesis in flowers and their industrialization. In: da Silva JAT (ed) Floriculture, ornamental and plant biotechnology, vol 1., Global science booksIsleworth, Middlesex, pp 619–628

    Google Scholar 

  • Okamura M, Nakayama M, Umemoto N, Cano EA, Hase Y, Nishizaki Y, Sasaki N, Ozeki Y (2013) Crossbreeding of a metallic color carnation and diversification of the peculiar coloration by ion-beam irradiation. Euphytica 191:45–56. doi:10.1007/s10681-012-0859-x

    Article  Google Scholar 

  • Radulescu I, Elmroth K, Stenerlöw B (2004) Chromatin organization contributes to non-randomly distributed doublestrand breaks after exposure to high-LET radiation. Radiat Res 161:1–8

    Article  CAS  PubMed  Google Scholar 

  • Rout GR, Das P (1997) Recent trends in the biotechnology of chrysanthemum: a critical review. Sci Hortic 69:239–256

    Article  Google Scholar 

  • Salamon MH (1980) A range-energy program for relativistic heavy ions in the region 1 < E<3000 MeV/amu. Report 10446, Lawrence Berkeley Laboratory, Berkeley, CA

  • Shikazono N, Yokota Y, Kitamura S, Suzuki C, Watanabe H, Tano S, Tanaka A (2003) Mutation rate and novel tt mutants of Arabidopsis thaliana induced by carbon ions. Genetics 163:1449–1455

    PubMed Central  CAS  PubMed  Google Scholar 

  • Shikazono N, Noguchi M, Fujii K, Urushibara A, Yokota A (2009) The yield, processing, and biological consequences of clustered DNA damage induced by ionizing radiation. J Radiat Res 50:27–36

    Article  CAS  PubMed  Google Scholar 

  • Sihver L, Schardt D, Kanai T (1998) Depth-dose distributions of high energy carbon, oxygen and neon beams in water. Jpn J Med Phys 18:1–21

    Google Scholar 

  • Skirvin RM, Janick J (1976) Tissue culture- induced variation in scented Pelargonium spp. J Amer Soc Hort Sci 101:281–290

    Google Scholar 

  • Stadler LJ (1928) Mutations in barley induced by X-rays and radium. Science 68:186–187

    Article  CAS  PubMed  Google Scholar 

  • Tanaka A (2012) Targeted mutation breeding of flower color by taking advantage of ion-beam irradiation and genomic information. Plant Biotechnol 29:191–192. doi:10.5511/plantbiotechnology.12.0002p

    Article  Google Scholar 

  • Tanaka Y, Sasaki N, Ohmiya A (2008) Biosynthesis of plant pigments: anthocyanins, betalains and carotenoids. Plant J 54:733–749

    Article  CAS  PubMed  Google Scholar 

  • Tanaka A, Shikazono N, Hase Y (2010) Studies on biological effects of ion beams on lethality, molecular nature of mutation, mutation rate, and spectrum of mutation phenotype for mutation breeding in higher plants. J Radiat Res 51:223–233. doi:10.1269/jrr.09143

    Article  CAS  PubMed  Google Scholar 

  • Torikoshi M, Minohara S, Kanematsu N, Komori M, Kanazawa M, Noda K, Miyahara N, Itoh H, Endo M, Kanai T (2007) Irradiation System for HIMAC. J Radiat Res 48:A15–A25

    Article  PubMed  Google Scholar 

  • The Joint FAO/IAEA Programme, mutant variety database (MVGS). http://mvgs.iaea.org/default.aspx

  • van Harten AM (1998) Mutation breeding, theory and practical applications. Cambridge University Press, New York

    Google Scholar 

  • Yamaguchi H, Shimizu A, Degi K, Morishita T (2008) Effects of dose and dose rate of gamma ray irradiation on mutation induction and nuclear DNA content in chrysanthemum. Breeding Sci 58:331–335

    Article  Google Scholar 

  • Yoshida H, Itoh Y, Ozeki Y, Iwashina T, Ymaguchi M (2004) Variation in chalcononaringenin 2′-O-glucoside content in the petals of carnations (Dianthus caryophyllus) bearing yellow flowers. Scientia Horiticulturae 99:175–186

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank Dr. Masayoshi Nakayama for useful discussions and the technical staff who established the ion beam irradiation system. This project was partly performed within the Heavy Ion Medical Accelerator in Chiba (HIMAC) co-operative research program (B340) at the National Institute of Radiological Sciences, Chiba, Japan.

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Authors and Affiliations

  1. Central Laboratories for Key Technologies, Kirin Company, Ltd., Fukuura 1-13-5, Kanazawa, Yokohama, Kanagawa, 236-0004, Japan

    Masachika Okamura

  2. Quantum Beam Science Center, Japan Atomic Energy Agency, Takasaki, Gunma, 370-1292, Japan

    Yoshihiro Hase & Atsushi Tanaka

  3. Research Center for Charged Particle Therapy, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage, Chiba, 263-8555, Japan

    Yoshiya Furusawa

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  1. Masachika Okamura
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  2. Yoshihiro Hase
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  3. Yoshiya Furusawa
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  4. Atsushi Tanaka
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Correspondence to Masachika Okamura.

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Okamura, M., Hase, Y., Furusawa, Y. et al. Tissue-dependent somaclonal mutation frequencies and spectra enhanced by ion beam irradiation in chrysanthemum. Euphytica 202, 333–343 (2015). https://doi.org/10.1007/s10681-014-1220-3

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  • DOI: https://doi.org/10.1007/s10681-014-1220-3

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