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A green and environmental sustainable approach to synthesis the Mn oxide nanomaterial from Punica granatum leaf extracts and its in vitro biological applications

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Abstract

Pathogenic fungal infections in fruit cause economic losses and have deleterious effects on human health globally. Despite the low pH and high water contents of vegetables and fresh, ripened fruits, they are prone to fungal and bacterial diseases. The ever-increasing resistance of phytopathogens toward pesticides, fungicides and bactericides has resulted in substantial threats to plant growth and production in recent years. However, plant-mediated nanoparticles are useful tools for combating parasitic fungi and bacteria. Herein, we synthesized biogenic manganese oxide nanoparticles (MnONPs) from an extract of Punica granatum (P. granatum), and these nanoparticles showed significant antifungal and antibacterial activities. The production of MnONPs from plant extracts was confirmed by infrared spectroscopy (FTIR), X-ray diffraction (XRD) and UV visible spectroscopy (UV). The surface morphology and shape of the nanoparticles were characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Using a detached fruit method, the MnONPs were shown to exhibit significant antimicrobial activities against two bacterial strains, E. coli and S. aureus, and against the fungal species P. digitatum. The results revealed that the MnONPs had a minimum antimicrobial activity at 25 µg/mL and a maximum antimicrobial activity at 100 µg/mL against bacterial strains in lemon (citrus). Furthermore, the MnONPs exhibited significant ROS scavenging activity. Finally, inconclusive results from the green-synthesized MnONPs magnified their significant synergetic effects on the shelf life of tomatoes (Lycopercicum esculantum) and indicated that they could be used to counteract the phytopathological effects of postharvest fungal diseases in fruits and vegetables. Overall, this method of MnONPs synthesis is inexpensive, rapid and ecofriendly. MnONPs can be used as potential antimicrobial agents against different microbial species.

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Data availability and material

Healthy equal size Fruit samples were purchased from fruit market. Nanoparticles were prepared in lab and their characterizations were performed in Shanghai Jiao tong University Analysis center with proper procedure. All nanomaterial procedure and applications data will be provided on reasonable request to Corresponding Author.

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Funding

This work was financial supported by Shanghai Science and Technology Commission (No. 21N21900200; 20392000300), Shanghai Agriculture Applied Technology Development Program (No. 20180203).

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  1. Muhammad Aamir Manzoor

    Present address: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China

Authors and Affiliations

  1. School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China

    Iftikhar Hussain Shah, Irfan Ali Sabir, Shazma Gulzar, Liying Chang & Yidong Zhang

  2. School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China

    Muhammad Aamir Manzoor

  3. National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China

    Muhammad Ashraf

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I H S conceptualization Formal analysis, Investigation, Methodology, Visualization, and Writing – original draft. M A M Conceptualization, Soft ware. I A S Formal analysis, Investigation, MA Formal analysis, Visualization, Data correction. S G acquisition of data, analysis and/or interpretation of data. L C Conceptualization, Writing – review & editing. Yidong Zhang: Supervision, Project administration, Writing – review & editing.. All authors read and approved the final manuscript.

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Shah, I.H., Manzoor, M.A., Sabir, I.A. et al. A green and environmental sustainable approach to synthesis the Mn oxide nanomaterial from Punica granatum leaf extracts and its in vitro biological applications. Environ Monit Assess 194, 921 (2022). https://doi.org/10.1007/s10661-022-10606-7

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