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The genetic differentiation of Colocasia esculenta growing in gold mining areas with arsenic contamination

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Abstract

Arsenic is a heavy metal found in contaminated gold mining areas and which can affect plant and animal species. This study aims to determine the concentration of As in the aquatic plant Colocasia esculenta as well as this plant’s genetic variability. Sediment and C. esculenta samples were collected from three studied sites at the edge of a stream around a gold mine. The arsenic concentrations in sediment and C. esculenta samples were analyzed using induction coupled plasma-mass spectrometry (ICP-MS). Genetic differentiations were studied by random amplified polymorphic DNA (RAPD) with dendrogram construction and analysis of genetic similarity (S). The results showed that the arsenic concentrations in sediment and C. esculenta samples ranged from 4.547 ± 0.318 to 229.964 ± 0.978 and 0.108 ± 0.046 to 0.406 ± 0.174 mg kg−1, respectively. To compare the samples studied to the reference site, RAPD fingerprints from 26 primers successfully produced 2301 total bands used for dendrogram construction and S value analysis. The dendrogram construction separates C. esculenta into four clusters corresponding to their sampling sites. The S values of the studied sample sites compared to the reference site are 0.676–0.779, 0.739–0.791, and 0.743–0.783 for sites 1, 2, and 3, respectively, whereas the values of the individuals within each site are as high as 0.980. These results suggest that As accumulation in aquatic plant species should be of concern because of the potential effects of As on aquatic plants as well as humans.

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Acknowledgments

This research was funded by Khon Kaen University under the Incubation Researcher Project, Genetics and Environmental Toxicology (GET) Research Group, and Integrated Water Resource Management Research and Development Center in Northeast Thailand, Khon Kaen University, Thailand.

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Authors and Affiliations

  1. Genetics and Environmental Toxicology (GET) Research Group, Khon Kaen University, Khon Kaen, Thailand

    Sirilak Boonmee & Lamyai Neeratanaphan

  2. Department of Environmental Science, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand

    Sirilak Boonmee & Lamyai Neeratanaphan

  3. Faculty of Environment and Resource Studies, Mahasarakham University, Maha Sarakham, 44150, Thailand

    Tawatchai Tanee

  4. Faculty of Science and Industrial Technology Prince, Songkla University, Suratthani Campus, Suratthani, 84000, Thailand

    Prodpran Khamon

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  1. Sirilak Boonmee
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  2. Lamyai Neeratanaphan
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Correspondence to Lamyai Neeratanaphan.

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Boonmee, S., Neeratanaphan, L., Tanee, T. et al. The genetic differentiation of Colocasia esculenta growing in gold mining areas with arsenic contamination. Environ Monit Assess 187, 227 (2015). https://doi.org/10.1007/s10661-015-4462-1

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  • DOI: https://doi.org/10.1007/s10661-015-4462-1

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