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Genotoxic effects of water pollution on two fish species living in Karasu River, Erzurum, Turkey

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Abstract

Karasu River, which is the only river in the Erzurum plain, is the source of the Euphrates River (Eastern Anatolia of Turkey). The river is in a serious environmental situation as a result of pollution by agricultural and industrial sewage and domestic discharges. The present study aims to evaluate genotoxic effects of toxic metals in chub, Leuciscus cephalus, and transcaucasian barb, Capoeta capoeta, collected from contaminated site of the Karasu River, in comparison with fish from an unpolluted reference site. Heavy metal concentrations in surface water of the river were determined. The condition factor (CF) was taken as a general biomarker of the health of the fish, and genotoxicity assays such as micronucleus (MN) and other nuclear abnormalities (NA) were carried out on the fish species studied. MN and NA such as kidney-shaped nucleus, notched nucleus, binucleated, lobed nucleus, and blebbed nucleus were assessed in peripheral blood erythrocytes, gill epithelial cells, and liver cells of the fish. A significant decrease in CF values associated with a significant elevation in MN and NA frequencies was observed in fish collected from the polluted sites compared with those from the reference site. Results of the current study show the significance of integrating a set of biomarkers to identify the effects of anthropogenic pollution. High concentrations of heavy metals have a potential genotoxic effects, and the toxicity is possibly related to industrial, agricultural, and domestic activities.

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Acknowledgments

The animal ethics committee permit for the study was received by Atatürk University Local Animal Ethics Committee (Number: B.30.2.ATA.0.23.85-99), and the rules of the committee were followed during the investigations. We thank three anonymous reviewers for their helpful comments and suggestions for improving the manuscript.

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  1. Science Faculty, Biology Department, Atatürk University, 25240, Erzurum, Turkey

    Zehra Yazıcı & Turgay Şişman

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  1. Zehra Yazıcı
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  2. Turgay Şişman
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Correspondence to Turgay Şişman.

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Yazıcı, Z., Şişman, T. Genotoxic effects of water pollution on two fish species living in Karasu River, Erzurum, Turkey. Environ Monit Assess 186, 8007–8016 (2014). https://doi.org/10.1007/s10661-014-3983-3

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  • DOI: https://doi.org/10.1007/s10661-014-3983-3

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