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Screening of indigenous entomopathogenic fungal isolates on plant parasitic nematodes in China

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Abstract

Root-knot nematodes (RKNs), including Meloidogyne spp., are renowned as one of the most destructive plant-parasites worldwide. The culture filtrates of entomopathogenic fungi (EPF), obtained through fermentation processes and containing significant enzyme and toxins, exhibit nematicidal activity. Moreover, the host specificity of EPF is notable, with significant variations in host spectrum and virulence among different strains. Thus, screening indigenous EPF against RKNs will broaden the resource base for developing biological control pesticides. In this study, we isolated 25 EPF strains through the Galleria bait method and investigated the pathogenicity against RKNs. Amongst the 25 EPF strains, which were molecularly identified as Beauveria spp., Metarhizium spp., Purpureocillium spp., and Cordyceps spp., several exhibited promising control effects on RKNs. Three EPF culture filters (B. bassiana 25, P. lilacinum 26, and M. pinghaeuse 39) exhibited nematicidal activity that was not affected by illumination methods or temperature. This research indicates the potential of B. bassiana 25, P. lilacinum 26, and M. pinghaeuse 39 as biological agents against RKNs.

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Data availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors gratefully acknowledge financial support: National Key R&D Program of China (2017YFE013040 and 2019YFE0120400), National Science Foundation (32171637) and Major Science and Technology Project (110202001034, LS-03).

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Authors and Affiliations

  1. College of Life Sciences, Nankai University, Tianjin, 300071, China

    Ming Fang, Jie Sun, Ailing Wang, Hongbo Tang, Lei Wang, Xianqin Wei & Weibin Ruan

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  1. Ming Fang
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  2. Jie Sun
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  3. Ailing Wang
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  4. Hongbo Tang
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  5. Lei Wang
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Correspondence to Xianqin Wei or Weibin Ruan.

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Fang, M., Sun, J., Wang, A. et al. Screening of indigenous entomopathogenic fungal isolates on plant parasitic nematodes in China. Eur J Plant Pathol (2024). https://doi.org/10.1007/s10658-024-02870-9

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