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Effectiveness of Lr34 gene in reducing leaf rust severity in wheat cultivar BRW 934 transferred through marker-assisted backcross

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Abstract

Leaf rust caused by Puccinia triticina Eriks. is one of the yield constraining diseases of wheat. It is prevalent in almost all wheat growing areas of the world. Durable leaf rust resistance conferred by integration of slow rusting and effective major genes is the best strategy to combat this disease. In the present study a slow rusting gene, Lr34 was transferred into a heat tolerant wheat variety BRW 934 using marker-assisted back crossing. The BRW 934 was found highly susceptible to several Indian pathotypes of P. triticina including 12–2, 77–1, 77–5, 77–7, 77–8, 77–9, 77–10 and 104–2. The selection of the Lr34 gene was carried out using STS (sequence tagged site) marker csLV34. BC1F1 plants carrying Lr34 gene showed leaf rust scores ranging from 5 to 20MR and rust score in the BRW 934 progenies without the Lr34 gene were 30S-100S. Presence of the Lr34 gene significantly reduced disease severity in the progenies. Maximum recovery (87.5%) of the recurrent parent genome was obtained in plant number 16 and 30 in the BC1F1 generation. These findings indicated that the Lr34 gene can be effectively used in developing leaf rust resistant wheat lines for the Eastern-Gangetic plains.

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Data that support the present findings can be made available on request to the corresponding author.

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Acknowledgements

Funding received from BAU, Sabour to CR is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Plant Breeding and Genetics, Bihar Agricultural University, Sabour, Bhagalpur, India

    Vipul Kumar Saini

  2. Department of Genetics and Plant Breeding, Agriculture University, Jodhpur, India

    Chandan Roy

  3. ICAR-Indian Institute of Wheat and Barley Research, Shimla, India

    Pramod Prasad

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  1. Vipul Kumar Saini
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  2. Chandan Roy
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Correspondence to Chandan Roy.

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Saini, V.K., Roy, C. & Prasad, P. Effectiveness of Lr34 gene in reducing leaf rust severity in wheat cultivar BRW 934 transferred through marker-assisted backcross. Eur J Plant Pathol (2024). https://doi.org/10.1007/s10658-024-02857-6

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