Abstract
Rhizomania caused by the Beet necrotic yellow vain virus (BNYVV) is the most damaging disease of sugar beet worldwide. Antibody-based resistance was used as an efficient strategy to generate transgenic sugar beet plants resistant to the virus through the expression of single-chain variable fragments (scFv) directed against a major coat protein of the virus, P21. Four anti-P21 scFv fusion proteins were targeted towards different subcellular compartments including the cytosol, apoplast, and mitochondrial outer membrane where P21 is probably located. Transgenic sugar beet plants showed levels of resistance correlated with the expression levels of scFv antibody when they were inoculated by rhizomania-infested soil. Although transgenic plants expressing scFv in various locations showed comparable titers of the virus, those expressing scFv targeted towards the apoplast or cytosol carrying the stabilizing KDEL tetrapeptide showed the highest rates of resistance against the virus. Further work may lead to rhizomania resistance.
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The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
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Acknowledgments
We would like to thank Dr. Mehdi Arbabi for kindly providing the single-chain variable fragment against the major coat protein of BNYVV. This work was funded by the National Institute of Genetic Engineering and Biotechnology of I.R. Iran grant No. 114 T.
Funding
This study was funded by the National Institute of Genetic Engineering and Biotechnology of I.R. Iran grant No. 114 T.
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Bajestani, M.J., Malboobi, M.A., Sabet, M.S. et al. Inhibition of beet necrotic yellow vein virus by the expression of a single-chain variable fragment antibody in sugar beet plants. Eur J Plant Pathol 164, 539–550 (2022). https://doi.org/10.1007/s10658-022-02578-8
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DOI: https://doi.org/10.1007/s10658-022-02578-8