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Inhibition of anthracnose symptoms in common bean by treatment of seeds with essential oils of Ocimum gratissimum and Syzygium aromaticum and eugenol

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Abstract

Essential oils (EOs) of Ocimum gratissimum and Syzygium aromaticum and eugenol were applied to common bean seeds harvested from plants with anthracnose symptoms, at a dose of 80 mg Kg−1 of EOs and 64 mg kg−1 of eugenol, using soybean oil as a vehicle. The treated seeds were planted in pots and grown in a greenhouse. Seeds treated with EOs and eugenol did not differ from the water and soybean oil controls with regard to the percentage seedling emergence. After treatment with EOs, only 21.6% and 28.6% of O. gratissimum and S. aromaticum plants developed symptoms. Following treatment with eugenol, a primary component in both EOs, disease symptoms occurred in only 19.6% of the plants, suggesting that eugenol is responsible for the fungicidal action of EOs of O. gratissimum and S. aromaticum. The chemical composition of EOs is unknown. The potential of EOs for controlling Colletotrichum lindemuthianum, responsible for anthracnose disease of common bean, is reported here for the first time.

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Acknowledgments

This work was supported by Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

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  1. Institute of Exact and Technological Sciences, Federal University of Viçosa, Campus Rio Paranaíba, P.O. box 38810-000, Rio Paranaíba, MG, Brazil

    Adriene Aparecida Silva & Geraldo Humberto Silva

  2. Department of Biology, Federal University of Lavras, P.O. box 37.200-900, Lavras, MG, Brazil

    Fernanda Aparecida Castro Pereira & Elaine Aparecida de Souza

  3. Department of Chemistry, Federal University of Lavras, P.O.box 37.200-900, Lavras, MG, Brazil

    Denilson Ferreira de Oliveira

  4. Laboratory for Processing Products of Plant Origin, Federal University of the Jequitinhonha and Mucuri - Campus JK, P.O.box 39100-000, Diamantina, MG, 39100-000, Brazil

    Danúbia Aparecida Costa Nobre

  5. Crop Physiology and Metabolism Laboratory, Federal University of Viçosa, Campus Rio Paranaíba, P.O.box 38810-000, Rio Paranaíba, Minas Gerais, Brazil

    Willian Rodrigues Macedo

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  1. Adriene Aparecida Silva
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Silva, A.A., Pereira, F.A.C., de Souza, E.A. et al. Inhibition of anthracnose symptoms in common bean by treatment of seeds with essential oils of Ocimum gratissimum and Syzygium aromaticum and eugenol. Eur J Plant Pathol 163, 865–874 (2022). https://doi.org/10.1007/s10658-022-02525-7

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