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Fusarium oxysporum f. sp. passiflorae isolates display variable virulence in Passiflora edulis Sims seedlings

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Abstract

Genetic variability among Fusarium oxysporum f. sp. passiflorae (FOP) isolates changes their aggressiveness pattern under controlled conditions. This study aimed at assessing the aggressiveness of FOP isolates in Passiflora edulis seedlings through survival analysis as well as anatomic analyses of infected roots. The study followed a completely randomized design with nine FOP isolates used to inoculate 30 P. edulis seedlings. Data were subjected to survival analysis based on Kaplan-Meier curves. The number of chlamydospores in the roots was counted through root clarification and microscopy. The incidence of wilted plants ranged from 20% to 90% of the isolates with an average of 50%. Isolate CMF3115 was the most aggressive with 70% of dead plants up to 70 d.a.i. and only 3% of plants surviving the disease. Although this outcome is possibly not correlated to mortality, this isolate was also responsible for large numbers of chlamydospores in roots. Thus, CMF3115 has the potential to be used for Passiflora germplasm screening under controlled conditions and may contribute to the selection of P. edulis cultivars resistant to FOP.

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Acknowledgments

The Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) provided a masters research grant to the first author (R.A.P.). The Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) provided a postdoctoral scholarship to the third author (L.K.S.L - PDJ 152109/2019-6), financial support (Process 421033/2018-5) and a research productivity fellowship to the second and last author (O.N.J - PQ312774/2018-4; F.F.L PQ309895/2016-2). The Embrapa Mandioca e Fruticultura research unit provided the plant material along with experimental, technical and financial support (Process 22.16.04.007.00.00).

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This work was funded by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Process#: 421033/2018–5) and macroprogram of Embrapa Mandioca e Fruticultura (Process#:22.16.04.007.00.00).

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Authors and Affiliations

  1. Universidade Federal do Recôncavo da Bahia, URFB, Cruz das Almas, Bahia, CEP 44380-000, Brazil

    Raoni Andrade Pires & Luana Nascimento Silva

  2. Embrapa Mandioca e Fruticultura, Box 007, Cruz das Almas, Bahia, CEP 44380-000, Brazil

    Onildo Nunes Jesus, Lucas Kennedy Silva Lima & Francisco Ferraz Laranjeira

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  1. Raoni Andrade Pires
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  2. Onildo Nunes Jesus
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  3. Lucas Kennedy Silva Lima
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  4. Luana Nascimento Silva
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  5. Francisco Ferraz Laranjeira
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Contributions

All authors contributed to writing, as well as toward interpreting the results, revising, and improving the paper. RAP and LNS performed the installation and conduct of the experiment and drafted the manuscript. LKSL, ONJ e FFL participated in the statistical analysis, and data interpretation. ONJ e FFL made corrections in the writing of the manuscript. LKSL, ONJ e FFL were the creators of the research.

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Correspondence to Francisco Ferraz Laranjeira.

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Pires, R.A., Jesus, O.N., Lima, L.K.S. et al. Fusarium oxysporum f. sp. passiflorae isolates display variable virulence in Passiflora edulis Sims seedlings. Eur J Plant Pathol 162, 465–476 (2022). https://doi.org/10.1007/s10658-021-02417-2

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