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Integrative taxonomy of Meloidogye ottersoni (Thorne, 1969) Franklin, 1971 (Nematoda: Meloidogynidae) parasitizing flooded rice in Brazil

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Abstract

A root-knot nematode (RKN) parasitizing rice (Oryza sativa L.) and causing damage in Santa Catarina (SC), Rio Grande do Sul (RS) and Paraná (PR) states (Brazil) was identified as Meloidogyne ottersoni (Thorne 1969) Franklin 1971. The species is redescribed from the Brazilian population from Meleiro (SC) and compared with the description of M. ottersoni from Wind Lake (Wisconsin, USA) with additional morphological, biochemical and molecular characterization. The female and male bear smaller stylets: 10-12 μm, 14-16 μm, respectively, when compared with M. graminicola: 12-14 μm, 16-18 μm, and M. oryzae: 14-16 μm, 18-20 μm. Meloidogyne ottersoni presents perineal patterns located on the contour of a slight protuberance. Striae are mostly continuous, never raised by transverse irregular striae, as frequently observed in M. graminicola and M. oryzae. Meloidogyne ottersoni belongs to the RKN group 11 described by Jepson (1987); the reproduction is by meiotic parthenogenesis and the somatic chromosome number is 18. The tail of second-stage juveniles is very long and thin, and tapers to a long, narrow, irregular hyaline terminus (M. ottersoni, 20.5 μm vs M. graminicola, 17.9 μm and M. oryzae, 22.0 μm, respectively). The ability of the Brazilian M. ottersoni population to parasitize canary grass, Phalaris arundinacea L. (type host), and barnyard grass, Echinocloa crus-galli, was confirmed. Biochemically, the esterase profile of M. ottersoni lacks any band (Est Ot0, Rm=0), which differentiates it from M. graminicola and M. oryzae (Est VS1, Rm=0.70 and Est O1, Rm=1.02, respectively). In Maximum Likelihood analysis of ITS, D2D3 and COXII-16S rRNA sequences, populations of M. ottersoni from different states of Brazil clustered together and were separated from other Meloidogyne spp., thus confirming that all four populations are very similar and conspecific.

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Acknowledgments

This work was supported by National Council for Scientific an Technological Development (CNPq), Embrapa Genetic Resources and Biotecnology, and Research Foundation of the Federal District (FAPDF). R.R. Leite and R.M.D.G. Carneiro thank CNPq and Coordination of Improvement of Higher Education (CAPES) for their scholarships and the assistant technician Ingrid Gracielle Martins da Silva for the electron microscopy photos and the Microanalysis Laboratory of the Institute of Biological Sciences of the University of Brasilia (LMM-UnB).

Funding

Funding provided by National Council for Scientific and Technological Development (CNPq), Embrapa Genetic Resources and Biotechnology and Research Foundation of the Federal District (FAPDF). Grant numbers:193.001.167/2015

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Author notes

  1. Raycenne Rosa Leite and Vanessa S. Mattos contributed equally to this work.

Authors and Affiliations

  1. Departamento de Fitopatologia, Universidade de Brasília, Brasília, DF, 70910-900, Brazil

    Raycenne Rosa Leite & Juvenil E. Cares

  2. Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, 70770901, Brazil

    Raycenne Rosa Leite, Vanessa S. Mattos, Ana Cristina M. M. Gomes, Daniela A. Souza & Regina M. D. G. Carneiro

  3. Rua Frei Humberto Weil, 29, Bairro Perequê, Porto Belo, SC, 88210-000, Brazil

    Leandro Grimaldi Py

  4. INRAE, CNRS, ISA, Université Nice Côte d’Azur, F-06903 Sophia Antipolis, Nice, France

    Philippe Castagnone-Sereno

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  1. Raycenne Rosa Leite
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Leite, R.R., Mattos, V.S., Gomes, A.C.M.M. et al. Integrative taxonomy of Meloidogye ottersoni (Thorne, 1969) Franklin, 1971 (Nematoda: Meloidogynidae) parasitizing flooded rice in Brazil. Eur J Plant Pathol 157, 943–959 (2020). https://doi.org/10.1007/s10658-020-02049-y

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