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Characterization of genetic diversity and pathogenicity of Phakopsora pachyrhizi mono-uredinial isolates collected in Brazil

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Abstract

The diversity of Phakopsora pachyrhizi populations has often resulted in the selection of virulent pathotypes following the release of rust-resistant cultivars. Thus, knowledge of the pathogenicity and variability of P. pachyrhizi specie, coupled with an understanding of its biology and host-pathogen interactions, is essential for better comprehension of the evolution and rapid adaption of the pathogen. Such comprehension can be useful for genetic breeding programs and other research areas. Therefore, this study aimed to assess the genetic variability and virulence among P. pachyrhizi mono-uredinial isolates obtained from field samples collected in Brazil during the period from 2007 to 2012 by comparing the sequences of Internal Transcribed Spacer (ITS) regions and AFLP markers and analyzing the responses of differential soybean genotypes. The polymorphisms among the AFLP markers (locally) and ITS sequences (globally) demonstrated the high genetic diversity of this pathogen. Phenotypic analysis revealed the presence of at least 16 different pathotypes present in soybean fields in Brazil. The most effective resistance gene was Rpp5 (PI 200487), followed by Rpp4 (PI 459025) and Rpp2 (230970). This study represents the first report on the virulence and molecular characterization of a collection of P. pachyrhizi mono-uredinial isolates, which might represent different races occurring in Brazilian fields in the period from 2007 to 2012.

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Acknowledgments

The authors would like to express their gratitude to all who provided assistance in collecting the material and conducting all the experiments. We greatly appreciate the financial support of CAPES, CNPq and Embrapa for this study.

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Authors and Affiliations

  1. Departamento de Agronomia, Programa de Pós-Graduação em Genética e 12 Melhoramento, Universidade Estadual de Maringá, Avenida Colombo, 5.790, 13, Maringá, PR, Caixa Postal 87020-900, Brazil

    Luana Mieko Darben & Adriana Gonela

  2. Empresa Brasileira de Pesquisa e Agropecuária- Embrapa Soja, Laboratório de 15 Biotecnologia Vegetal e Bioinformática, Rodovia Carlos João Strass – Distrito de 16 Warta, Londrina, PR, Caixa Postal 231, 86001-970, Brazil

    Luana Mieko Darben, Alessandra Yokoyama, Fernanda Machado Castanho, Valéria Stefania Lopes-Caitar, Cláudia Vieira Godoy & Francismar Corrêa Marcelino-Guimarães

  3. Departamento de Bioquímica e Biotecnologia, Programa de Pós-Graduação em 18 Biotecnologia, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid (PR 19 445) - km 380, Londrina, PR, Caixa Postal 10.011, 86057-970, Brazil

    Alessandra Yokoyama

  4. Centro de Ciências Agrárias, Programa de Mestrado em Agronomia, Universidade 21 Estadual do Norte do Paraná, Campus Luiz Meneghel de Bandeirantes, Rodovia BR22 369 Km 54, Bandeirantes, PR, Brazil

    Fernanda Machado Castanho, Mayra Costa da Cruz Gallo de Carvalho & Sandremir de Carvalho

  5. Departamento de Biologia Geral, Programa de Pós-Graduação em Genética e 24 Biologia Molecular, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid 25 (PR 445) - km 380, Londrina, PR, Caixa Postal 10.011, 86057-970, Brazil

    Valéria Stefania Lopes-Caitar

  6. Empresa Brasileira de Pesquisa e Agropecuária, Embrapa Soja, Laboratório de 34 Biotecnologia Vegetal e Bioinformática, Rodovia Carlos João Strass – Distrito de 35 Warta, Londrina, PR, Caixa Postal 231, 86001-970, Brazil

    Francismar Corrêa Marcelino-Guimarães

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  1. Luana Mieko Darben
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  2. Alessandra Yokoyama
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  3. Fernanda Machado Castanho
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  9. Francismar Corrêa Marcelino-Guimarães
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Correspondence to Francismar Corrêa Marcelino-Guimarães.

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Darben, L.M., Yokoyama, A., Castanho, F.M. et al. Characterization of genetic diversity and pathogenicity of Phakopsora pachyrhizi mono-uredinial isolates collected in Brazil. Eur J Plant Pathol 156, 355–372 (2020). https://doi.org/10.1007/s10658-019-01872-2

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