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Botryosphaeriaceae species associated with blueberry stem blight and dieback in the Centre Region of Portugal

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Abstract

Blueberry (Vaccinium corymbosum) cultivation in Portugal has been growing considerably in the last decade, making it a highly profitable fruit crop. Species of Botryosphaeriaceae are relevant pathogens of blueberry causing stem blight and dieback. In this study a survey was carried out in blueberry orchards in the centre region of Portugal in order to assess which species of Botryosphaeriaceae are associated with stem blight and dieback symptoms. Their presence as endophytes was also evaluated in asymptomatic plants. Four species were identified, Botryosphaeria dothidea, Neofusicoccum parvum, N. australe and N. eucalyptorum. Of these, B. dothidea and N. parvum were the most commonly found. Pathogenicity trials showed that the four species tested were pathogenic to blueberry plants and revealed that N. parvum, was the most aggressive species, and most likely the main causal agent of blueberry stem blight and dieback in Portugal. Neofusicoccum eucalyptorum, a common Eucalyptus pathogen, is reported for the first time as a pathogen of blueberry. This represents a significant host jump whose potential future effects on blueberry plantations are not fully understood.

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References

  • Alves, A., Correia, A., Luque, J., & Phillips, A. (2004). Botryosphaeria corticola, sp. nov. on Quercus species, with notes and description of Botryosphaeria stevensii and its anamorph, Diplodia mutila.Mycology, 96(3), 598–613.

    Article  Google Scholar 

  • Alves, A., Crous, P. W., Correia, A., & Phillips, A. J. L. (2008). Morphological and molecular data reveal cryptic speciation in Lasiodiplodia theobromae. Fungal Diversity, 28, 1–13.

    Google Scholar 

  • Alves, A., Phillips, A. J. L., Henriques, I., & Correia, A. (2007). Rapid differentiation of species of Botryosphaeriaceae by PCR fingerprinting. Research in Microbiology, 158, 112–121.

    Article  CAS  Google Scholar 

  • Barradas, C., Phillips, A. J. L., Correia, A., Diogo, E., Bragança, H., & Alves, A. (2016). Diversity and potential impact of Botryosphaeriaceae species associated with Eucalyptus globulus plantations in Portugal. European Journal of Plant Pathology, 146(2), 245–257.

    Article  Google Scholar 

  • Boyzo-Marin, J., Rebollar-Alviter, A., Silva-Rojas, H. V., & Ramirez-Maldonaldo, G. (2016). First report of Neofusicoccum parvum causing stem blight and dieback of blueberry in Mexico. Plant Disease, 100(12), 2424.

    Article  Google Scholar 

  • Burgess, T. I., Tan, Y. P., Garnas, J., Edwards, J., Scarlett, K. A., Shuttleworth, L. A., Daniel, R., Dann, E. K., Parkinson, L. E., Dinh, Q., Shivas, R. G., & Jami, F. (2018). Current status of the Botryosphaeriaceae in Australia. Australasian Plant Pathology, 48(1), 35–44.

    Article  Google Scholar 

  • Carbone, I., & Kohn, L. M. (1999). A method for designing primer set for speciation studies in filamentous ascomycetes. Mycologia, 91, 553–556.

    Article  CAS  Google Scholar 

  • Castillo, S., Borrero, C., Castaño, R., Rodríguez, A., & Avilés, M. (2013). First report of canker disease caused by Neofusicoccum parvum and N. australe on blueberry bushes in Spain. Plant Disease, 97(8).

  • Choi, I. Y. (2011). First report of bark dieback on blueberry caused by Botryosphaeria dothidea in Korea. Plant Disease, 95(2), 227.

    Article  CAS  Google Scholar 

  • Choi, I. Y., Sharma, P. K., & Cheong, S. S. (2012). First Report of Neofusicoccum parvum associated with bark dieback of blueberry in Korea. The Plant Pathology Journal, 28(2), 217.

  • Creswell, T. C., & Milholland, R. D. (1987). Responses of blueberry genotypes to infection by Botryosphaeria dothidea. Plant Disease, 71(8), 710–713.

  • Espinoza, J. G., Briceño, E. X., Chávez, E. R., Úrbez-Torres, J. R., & Latorre, B. A. (2009). Neofusicoccum spp. associated with stem canker and dieback of blueberry in Chile. Plant Disease, 93(11), 1187–1194.

  • FAOSTAT. (2019). Food and Agriculture Organization of the United Nations. http://www.fao.org/faostat/en/#home. Accessed 29 March 2019.

  • Fonseca, L. L. & Oliveira, P. B. (2007). A planta de mirtilo: Morfologia e Fisiologia. Instituto Nacional de Investigação Agrária e Veterinária. http://www.iniav.pt/fotos/gca/2_a_planta_de_mirtilo_morfologia_e_fisiologia_1369130315.pdf. Accessed 29 March 2019.

  • Hall, T. A. (1999). BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.

    CAS  Google Scholar 

  • Hancock, J. F., & Retamales, J. B. (2012). Blueberries. USA: CAB International.

    Book  Google Scholar 

  • INE. (2019). Portal do Instituto Nacional de Estatística: Estatísticas agrícolas 2018. http://www.ine.pt/. Accessed 19 August 2019.

  • Koike, S. T., Rooney-Latham, S., & Wright, A. F. (2014). First report of stem blight of blueberry in California caused by Neofusicoccum parvum. Plant Disease, 98(9), 1280–1280.

    Article  CAS  Google Scholar 

  • Kong, C. S., Qiu, L. X., Yu, F. X., & Yu, L. (2010). First report of Neofusicoccum vitifusiforme causing blueberry blight of blueberry in China. Plant Disease, 94(11), 1373.

    Article  CAS  Google Scholar 

  • Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution, 35, 1547–1549.

    Article  CAS  Google Scholar 

  • Larkin, M. A., Blackshields, G., Brown, N. P., Chenna, R., Gettigan, P. A., William, H., & Higgins, D. G. (2007). Clustal W and Clustal X version 2.0. Bioinformatics, 23, 2947–2948.

    Article  CAS  Google Scholar 

  • Liddle, R. L., Akinsanmi, O. A., & Galea, V. J. (2019). Non-host specificity of Botryosphaeriaceae on macadamia and blueberry. Australasian Plant Pathology, 48, 65–73.

    Article  CAS  Google Scholar 

  • Lopes, A., Barradas, C., Phillips, A. J. L., & Alves, A. (2016). Diversity and phylogeny of Neofusicoccum species occurring in forest and urban environments in Portugal. Mycosphere, 7, 906–920.

    Article  Google Scholar 

  • Lopes, A., Phillips, A. J. L., & Alves, A. (2017). Mating type genes in the genus Neofuscoccum: Mating strategies and usefulness in species delimitation. Fungal Biology, 121(4), 394–404.

  • Ma, L., Sun, Z., Zeng, Y., Luo, M., & Yang, Z. (2018). Molecular mechanism and health role of functional ingredients in blueberry for chronic disease in human beings. International Journal of Molecular Sciences, 19(9), 2785.

    Article  Google Scholar 

  • Madeira, B. S. P. (2016). Cultura do Mirtilo. Portugal: Agrobook.

    Google Scholar 

  • Mehl, J. W. M., Slippers, B., Roux, J., & Wingfield, M. J. (2017). Overlap of latent pathogens in the Botryosphaeriaceae on a native and agricultural host. Fungal Biology, 121(4), 405–419.

    Article  Google Scholar 

  • Milholland, R. D. (1972). Histopathology and pathogenicity of Botryosphaeria dothidea on blueberry stems. Phytopathology, 62, 654–660.

    Article  Google Scholar 

  • Milholland, R. D. (1984). Occurrence of a new race of Botryosphaeria corticis on highbush and rabbiteye blueberry. Plant Disease, 68, 522–523.

    Article  Google Scholar 

  • Osorio, J. A., Crous, C. J., de Beer, Z. W., Wingfield, M. J., & Roux, J. (2017). Endophytic Botryosphaeriaceae, including five new species, associated with mangrove trees in South Africa. Fungal Biology, 121, 361–393.

    Article  Google Scholar 

  • Pavlic, D., Slippers, B., Coutinho, T. A., & Wingfield, M. J. (2007). Botryosphaeriaceae occurring on native Syzygium cordatum in South Africa and their potential threat to Eucalyptus. Plant Pathology, 56, 624–636.

    Article  CAS  Google Scholar 

  • Pavlic, D., Wingfield, M., Boissin, E., & Slippers, B. (2015). The distribution of genetic diversity in the Neofusicoccum parvum/N. ribis complex suggests structure correlated with level of disturbance. Fungal Ecology, 13(3), 93–102.

  • Pérez, S. F., & Guerrero, J. C. (2014). Detection of Neofusicoccum nonquaesitum causing dieback and canker in highbush blueberry from Southern Chile. Journal of Soil Science and Plant Nutrition, 14(3), 581–588.

  • Pérez, C. A., Wingfield, M. J., Slippers, B., Altier, N. A., & Blanchette, R. A. (2010). Endophytic and canker-associatedBotryosphaeriaceae occurring on non-nativeEucalyptus and native Myrtaceae trees in Uruguay. Fungal Diversity, 41, 53–69.

  • Phillips, A. J. L., Alves, A., Abdollahzadeh, J., Slippers, B., Wingfield, M. J., Groenewald, J. Z., & Crous, P. W. (2013). The Botryosphaeriaceae: Genera and species known from culture. Studies in Mycology, 76, 51–167.

    Article  CAS  Google Scholar 

  • Phillips, A. J. L., Oudemans, P. V, Correia, A., & Alves, A. (2006). Characterisation and epitypification of Botryosphaeria corticis, the cause of blueberry cane canker. Fungal Diversity, 21, 141–155.

  • Phillips, A. J. L., Rumbos, I. C., Alves, A., & Correia, A. (2005). Morphology and phylogeny of Botryosphaeria dothidea causing fruit rot of olives. Mycopathologia, 159(3), 433–439.

    Article  CAS  Google Scholar 

  • Scarlett, K. A., Shuttleworth, L. A., Collins, D., Rothwell, C. T., Guest, D. I., & Daniel, R. (2018). Botryosphaeriales associated with stem blight and dieback of blueberry (Vaccinium spp.) in New South Wales and Western Australia. Australasian Plant Pathology, 48, 45–57.

  • Sessa, L., Abreo, E., & Lupo, S. (2018). Diversity of fungal latent pathogens and true endophytes associated with fruit trees in Uruguay. Journal of Phytopathology, 166, 633–647.

    Article  Google Scholar 

  • Slippers, B., Crous, P. W., Jami, F., Groenewald, J. Z., & Wingfield, M. J. (2017). Diversity in the Botryosphaeriales: Looking back, looking forward. Fungal Biology, 121(4), 307–321.

    Article  Google Scholar 

  • Slippers, B., & Wingfield, M. J. (2007).Botryosphaeriaceae as endophytes and latent pathogens of woody plants: diversity, ecology and impact. Fungal Biology Reviews, 21(2–3), 90–106.

    Article  Google Scholar 

  • Stukenbrock, E. H. (2013). Evolution, selection and isolation: A genomic view of speciation in fungal plant pathogens. New Phytologist, 199(4), 895–907.

    Article  Google Scholar 

  • Tennakoon, K. M. S., Ridgway, H. J., Jaspers, M. V., & Jones, E. E. (2017). Botryosphaeriaceae species associated with blueberry dieback and sources of primary inoculum in propagation nurseries in New Zealand. European Journal of Plant Pathology, 150, 363–374.

    Article  Google Scholar 

  • Triki, M. A., Krid HadjTaieb, S., Cheffi, M., Gharbi, Y., & Rhouma, A. (2015). First report of dieback of olive trees caused by Neofusicoccum australe in Tunisia. Journal of Plant Pathology, 97(1), 212.

    Google Scholar 

  • Úrbez-Torres, J. R., & Gubler, W. D. (2009). Pathogenicity of Botryosphaeriaceae species isolated from grapevine cankers in California. Plant Disease, 93, 584–592.

    Article  Google Scholar 

  • Úrbez-Torres, J. R. (2011). The status of Botryosphaeriaceae species infecting grapevines. Phytopathologia Mediterranea, 50, 5–45.

    Google Scholar 

  • Vaidya, G., Lohman, D. J., & Meier, R. (2010). SequenceMatrix: Concatenation software for the last assembly of multi-gene datasets with character set and codon information. Cladistics, 27(2), 171–180.

    Article  Google Scholar 

  • White, T., J., Bruns, T. D., Lee, S. B., & Taylor, J. W. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M.A. Innis (Ed.), PCR Protocols: a guide to methods and applications (pp. 315–322). San Diego: Academic Press.

  • Wright, F., & Harmon, P. F. (2010). Identification of species in the Botryosphaeriaceae family causing stem blight on southern highbush blueberry in Florida. Plant Disease, 94(8), 966–971.

    Article  CAS  Google Scholar 

  • Wright, E. R., Mandolesi, A., Rivera, M. C., Pérez, B. A., Mezzetti, B., & Oliveira, P. B. (2012). Neofusicoccum parvum, blueberry pathogen in Argentina. Acta Horticulturae, 926, 619–623.

  • Xu, C., Zhang, H., Zhou, Z., Hu, T., Wang, S., Wang, Y., & Cao, K. (2015). Identification and distribution of Botryosphaeriaceae species associated with blueberry stem blight in China. European Journal of Plant Pathology, 143(4), 737–752.

  • Yu, L., Impaprasert, R., Zhao, J. R., Xu, S. G., & Wu, X. (2013). Stem die-back of highbush blueberries caused by Neofusicoccum parvum in China. New diseases reports, 27(3), 5197.

    Google Scholar 

  • Yu, L., Rarisara, I., Xu, G. S., Wu, X., & Zhao, J. R. (2012). First report of stem blight of blueberry caused by Botryosphaeria dothidea in China. Plant Disease, 96(11), 1697.

    Article  CAS  Google Scholar 

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Acknowledgments

The Portuguese Foundation for Science and Technology (FCT/MCTES) finances CESAM (UID/AMB/50017/2019) through national funds and the PhD grant of Sandra Hilário (SFRH/BD/137394/2018). Liliana Santos is funded by national funds (OE), through FCT in the scope of the framework contract foreseen in the numbers 4, 5 and 6 of the article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19.

The authors are thankful to Mirtilusa, Lda for providing the blueberry plants used for pathogenicity tests and to Sandro Alves and Custódio Daniel Borges for supplying the plant material from their plantations.

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  1. Departamento de Biologia, CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal

    Sandra Hilário, Anabela Lopes, Liliana Santos & Artur Alves

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  1. Sandra Hilário
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  2. Anabela Lopes
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Correspondence to Artur Alves.

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Hilário, S., Lopes, A., Santos, L. et al. Botryosphaeriaceae species associated with blueberry stem blight and dieback in the Centre Region of Portugal. Eur J Plant Pathol 156, 31–44 (2020). https://doi.org/10.1007/s10658-019-01860-6

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