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Assessing the Belgian potato Alternaria population for sensitivity to fungicides with diverse modes of action

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Abstract

Early blight and brown spot, caused by respectively Alternaria solani and Alternaria alternata, can lead to severe yield losses in potato-growing areas. To date, fungicide application is the most effective measure to control the disease. However, in recent years, a reduced sensitivity towards several active ingredients has been reported. To shed light on this issue, Alternaria isolates were collected from different potato fields in Belgium during two growing seasons. Subsequently, the sensitivity of these isolates was assessed using four widely used fungicides with different modes of action. Demethylation inhibitors, quinone outside inhibitors, a dithiocarbamate and a carboxylic acid amide were included in this study. Although all fungicides reduced spore germination and vegetative growth of Alternaria species to some extent, the interspecies sensitivity was very variable. In general, A. solani was more suppressed by the fungicides compared to A. alternata. The effectiveness of the dithiocarbamate mancozeb was high, whereas the quinone outside inhibitor azoxystrobin showed a limited activity, especially towards A. alternata. Therefore, a subset of the A. alternata and A. solani isolates was tested for the presence of, respectively, the G143A substitution and the F129L substitution in the cytochrome b. The frequency of A. alternata isolates bearing the resistant G143A allele (approximately 65%) was comparable in both sampling years, although sensitivity of isolates decreased during the growing season. This finding points to a shift of the population towards resistant isolates. Both the European genotype I and American genotype II were present in the A. solani population, with genotype I being the most prevalent. None of the genotype I isolates carried the F129L substitution, whereas in 83% of the genotype II isolates this substitution was present. Our results demonstrate for the first time that the Belgian Alternaria population on potato comprises a considerable broad spectrum of isolates with different sensitivity to fungicides.

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Acknowledgments

This work was financially supported by the Agency for Innovation by Science and Technology (IWT, Brussels, Belgium). Furthermore, we are thankful to the people of Inagro and the Agricultural center for Potato research for the participation in collecting field samples.

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Authors and Affiliations

  1. Department of Applied Bioscience Engineering, Faculty of Bioscience Engineering, Ghent University, Valentin Vaerwyckweg 1, 9000, Ghent, Belgium

    S. Landschoot, M. Vandecasteele, J. Carrette, K. Audenaert & G. Haesaert

  2. KERMIT, Department of Mathematical Modelling, Statistics and Bioinformatics, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, 9000, Ghent, Belgium

    B. De Baets

  3. Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, 9000, Ghent, Belgium

    M. Höfte

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  1. S. Landschoot
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  2. M. Vandecasteele
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  4. B. De Baets
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Correspondence to S. Landschoot.

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S. Landschoot and M. Vandecasteele contributed equally to this work.

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Landschoot, S., Vandecasteele, M., Carrette, J. et al. Assessing the Belgian potato Alternaria population for sensitivity to fungicides with diverse modes of action. Eur J Plant Pathol 148, 657–672 (2017). https://doi.org/10.1007/s10658-016-1123-3

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