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Confirmation of Peronospora agrimoniae as a distinct species

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Abstract

Leaves with typical symptoms of downy mildew were found on common agrimony in the Czech Republic in 2014 and 2015 and at several locations in Germany from 2010 to 2014. The causal agent of downy mildew of agrimony was often reported as Peronospora agrimoniae, but sometimes also as P. sparsa. Morphological characteristics of the pathogens found in both countries are in the range of previous works for P. agrimoniae, but also other downy mildews parasitic on Rosaceae, rendering their discrimination based on published observations difficult. For molecular identification sequencing of several loci (nrITS rDNA, cox1 and cox2) was performed. Phylogenetic analyses based on nrITS rDNA clearly separated P. agrimoniae from other Peronospora species infecting Rosaceae. Thus, considering P. agrimoniae as separate species seems justified. Two German specimens were identical to two Czech samples in both nrITS rDNA and cox1 mtDNA sequences, but differed in a single nucleotide substitution in cox2 region. To our knowledge, this is the first verified record of P. agrimoniae on common agrimony in the Czech Republic.

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Acknowledgments

Research was supported by: grant No. LO1204 (Sustainable development of research in the Centre of the Region Haná) from the National Program of Sustainability I, MEYS, Czech Republic; Internal Grant Agency of Palacký University in Olomouc, the Czech Republic (IGA 2016-001); fellowship from the Alexander von Humboldt foundation; LOEWE, Landesinitiative zur Entwicklung wissenschaftlich-ökonmischer Exzellenz, a funding program of the Government of Hessen, Germany, in the framework of the IPF—Integrative Fungal Research Cluster and the BiK-F—Biodiversity and Climate Research Centre. Authors thank to Prof. Aloisie Poulíčková for providing DIC microscopy photograph of P. agrimoniae conidiophore.

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Authors and Affiliations

  1. Centre of the Region Haná for Biotechnological and Agricultural Research, Section of Applied Research of Vegetables and Special Crops of the Crop Research Institute, Department of Genetic Resources for Vegetables, Medicinal and Special Plants, Šlechtitelů 29, Olomouc-Holice, CZ-78371, Czech Republic

    Irena Petrželová, Michaela Jemelková & Ivana Doležalová

  2. Biodiversity and Climate Research Centre (BiK-F) at the Senckenberg Gesellschaft für Naturforschung, Senckenberganlage 25, D-60325, Frankfurt am Main, Germany

    Young-Joon Choi, Julia Kruse & Marco Thines

  3. Department of Biological Sciences, Institute of Ecology, Evolution and Diversity, Goethe University Frankfurt am Main, Max-von-Laue-Str. 9, D-60438, Frankfurt am Main, Germany

    Young-Joon Choi, Julia Kruse & Marco Thines

  4. Department of Biology, Kunsan National University, 573-701, Miryong-Dong, Gunsan, Korea

    Young-Joon Choi

  5. Integrative Fungal Research Cluster (IPF), Georg-Voigt-Str. 14-16, D-60325, Frankfurt am Main, Germany

    Marco Thines

  6. Faculty of Science, Department of Botany, Palacký University in Olomouc, Šlechtitelů 27, Olomouc, CZ-78371, Czech Republic

    Miloslav Kitner

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  1. Irena Petrželová
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Petrželová, I., Choi, YJ., Jemelková, M. et al. Confirmation of Peronospora agrimoniae as a distinct species. Eur J Plant Pathol 147, 887–896 (2017). https://doi.org/10.1007/s10658-016-1058-8

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