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PGPM-induced defense-related enzymes in aerobic rice against rice leaf blast caused by Pyricularia oryzae

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Abstract

Rice blast caused by Pyricularia oryzae is the most devastative disease especially under aerobic cultivation systems. The bio-efficacy of plant growth-promoting microorganisms: Pseudomonas aeruginosa (UPMP1), Corynebacterium agropyri (UPMP7), Enterobacter gergoviae (UPMP9) and Bacillus amyloliquefaciens (UPMS3), Trichoderma harzianum (UPMT1) and Trichoderma virens (UPMT2) in induction of defense-related enzymes against Pyricularia oryzae was evaluated in rice cultivated under aerobic conditions. Under dual culture plate testing, all PGPMs indicated antagonism against P. oryzae with percentage inhibition radial growth (PIRG) which ranged from 51.69–81.97 %. The bio-efficacy of the respective PGPM in induction of defense-related enzymes in rice seedlings was evaluated based on individual inoculation before challenged inoculation with P. oryzae under greenhouse conditions. Inoculation of all PGPMs significantly reduced rice leaf blast severity at day eight after P. oryzae inoculation. The reduction in rice leaf blast disease severity was associated to the increase of peroxidase (PO), polyphenol oxidase (PPO) and phenylalanine ammonia-lyase (PAL) activities in rice seedlings when pre-inoculated with PGPMs. The highest leaf blast disease reduction (59.17 %) occurred with rice seedlings pre-inoculated with C. agropyri (UPMP9), followed by P. aeruginosa (UPMP1) (40.65 %), T. harzianum (UPMT1) (42.23 %), T. virens (UPMT2) (20.85 %), E. gergoviae (UPMP9) (17.84 %) and B. amyloliquefaciens (UPMS3). The high efficiency of PGPM in leaf blast disease suppression was associated with significant increase in total microbial activity (FDA hydrolysis) in rhizosphera soil (4.80–7.86 μg/g/0.5 h) compared to the control (2.25 μg/g/0.5 h). Thus, the application of PGPM is a potential alternative approach in rice leaf blast disease management of aerobic rice.

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Acknowledgments

The authors acknowledge the Ministry of Science, Technology and Innovation (MOSTI) for the research grant administered through the Science Fund of MOA (05-01-24-SF 1034), the School of Food Science and Technology, Universiti Malaysia Terengganu and the Ministry of Higher Education Malaysia through a doctorial fellowship and the Institute of Tropical Agriculture and the Faculty of Agriculture, Universiti Putra Malaysia for providing the research facilities.

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Authors and Affiliations

  1. Laboratory of Food Crops, Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia

    L. C. Ng, M. Sariah & O. Radziah

  2. School of Food Science and Technology, Universiti Malaysia Terengganu, 21030, Kuala Terengganu, Terengganu, Malaysia

    L. C. Ng

  3. Malaysian Agriculture Research and Development Institute, P.O. Box 12301, General Post Office, 50774, Kuala Lumpur, Malaysia

    O. Sariam

  4. Department of Land Management, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia

    O. Radziah

  5. Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia

    M. Sariah & M. A. Zainal Abidin

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  1. L. C. Ng
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Correspondence to L. C. Ng.

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Ng, L.C., Sariah, M., Sariam, O. et al. PGPM-induced defense-related enzymes in aerobic rice against rice leaf blast caused by Pyricularia oryzae . Eur J Plant Pathol 145, 167–175 (2016). https://doi.org/10.1007/s10658-015-0826-1

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  • DOI: https://doi.org/10.1007/s10658-015-0826-1

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