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Control of soil-borne pathogen Fusarium oxysporum by biological soil disinfestation with incorporation of various organic matters

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Abstract

Banana Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (FOC) is a worldwide soil-borne disease that causes serious economic losses every year. Effective ways of preventing the disease are still not available. In this study, biological soil disinfestation (BSD) with incorporation of various organic matters under flooding conditions combined with plastic mulching was applied to suppress FOC and prevent banana Fusarium wilt disease. The biological properties of the soil during the BSD process were investigated using quantitative real-time PCR and denaturing gradient gel electrophoresis. The results showed that the values of soil redox potential significantly decreased by >800 mV in the treatments that incorporated rice straw and bagasse under flooding conditions compared with untreated soil. The lowest soil pH (5.36) and a significant decline in the FOC population to 2.79 % of untreated soil were obtained in flooded soil with the highest amount of rice straw. Incorporation of bagasse, pig manure, and lime also significantly decreased the populations of FOC, but were less effective than rice straw. Application of rice straw under flooding conditions significantly increased soil bacterial diversity, and the increased bacteria were considered to have an antagonistic effect on FOC. In 2013 field experiment, a wilt disease control efficiency of 82.3 % was obtained in flooded soil incorporated with 0.5 % (w/w) rice straw compared with untreated soil, and the control efficiency of BSD was further confirmed in 2014 field experiment. These results indicated that BSD can reduce FOC populations in soil, ameliorate soil microbial communities, and reduce the occurrence of banana Fusarium wilt disease.

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Acknowledgments

This study was financially supported by the National Natural Science Foundation of China (Grant Nos. 41301335, 41330744), the Specialized Research Fund for the Doctoral Program of Higher Education of China (20133207120018), China Postdoctoral Science Foundation (2014M551622), the Natural Science Foundation of Jiangsu Province (BK20140062), the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (13KJA210002), Outstanding innovation team in Colleges and universities in Jiangsu Province and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. School of Geography Science, Nanjing Normal University, Nanjing, China

    Xin Qi Huang, Teng Wen, Jin Bo Zhang, Tong Bin Zhu, Liang Liang Liu & Zu Cong Cai

  2. Jiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, Nanjing Normal University, Nanjing, China

    Xin Qi Huang, Teng Wen, Jin Bo Zhang & Zu Cong Cai

  3. Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, Nanjing, China

    Xin Qi Huang & Zu Cong Cai

  4. Key Laboratory of Vitual Geographical Environment (VGE), Ministry of Education, Nanjing Normal University, Nanjing, China

    Xin Qi Huang, Jin Bo Zhang & Zu Cong Cai

  5. College of Agriculture, Hainan University, Haikou, China

    Lei Meng

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  1. Xin Qi Huang
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  2. Teng Wen
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  3. Jin Bo Zhang
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Correspondence to Zu Cong Cai.

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Huang, X.Q., Wen, T., Zhang, J.B. et al. Control of soil-borne pathogen Fusarium oxysporum by biological soil disinfestation with incorporation of various organic matters. Eur J Plant Pathol 143, 223–235 (2015). https://doi.org/10.1007/s10658-015-0676-x

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