Abstract
A total of 80 single–lesion isolates of Phytophthora infestans were collected from tomatoes and potatoes in several locations in Chiang Mai and Tak provinces in 2000–2002. These isolates were analyzed for mating type, metalaxyl sensitivity, mitochondrial DNA (mtDNA) haplotype RFLP pattern as determined by probe RG57, and for microsatellite markers. All isolates were A1 mating type. Isolates from tomato were usually sensitive to metalaxyl, but isolates from potato were usually resistant to metalaxyl. With one exception, all tomato isolates were related to the US-1 clonal lineage. With two exceptions, all potato isolates were related to two European lineages. In these two provinces, the populations of P. infestans on tomatoes are clearly different from those on potatoes.
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Acknowledgments
The authors gratefully acknowledge the Center of Excellence on Agricultural Biotechnology, Science and Technology Postgraduate Education and Research Development Office, Office of Higher Education Commission, Ministry of Education (AG-BIO/PERDO-CHE), for Ph. D. grant. We are also grateful for the National Center for Genetic Engineering and Biotechnology for the research and Ph. D. grants. The Tropical Vegetable Research Center (TVRC), Kasetsart University, and Tomato Genetic Center, UC Davis, for tomato seeds and we thank Dr. W. Stevenson of the University of Wisconsin-Madison for the P. infestans standard isolates, Dr. W. E. Fry of Cornell University for providing the RG57 probe and the P. infestans standard isolates, Associate Professor Dr. Wichai Kositratana and Dr. David Hole for their valuable comments. We thank Mr. Michael Cooper for English proof reading of the manuscript.
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Petchaboon, K., Srisawad, N., Pongam, P. et al. Phenotypic and genotypic structure of Phytophthora infestans populations on tomato and potato in the North of Thailand in 2000–2002. Eur J Plant Pathol 140, 441–454 (2014). https://doi.org/10.1007/s10658-014-0479-5
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DOI: https://doi.org/10.1007/s10658-014-0479-5