Abstract
Stem rot symptoms caused by pectinolytic bacteria of Genus Pectobacterium and Genus Dickeya, which are commonly referred to as blackleg, strongly impact the quality of seed potato production in most European countries. Several biotic and abiotic factors, such as cultivar susceptibility, isolate aggressiveness, mother tuber infection density and a wide range of soil-related and climatic factors have been identified in the literature as having an effect on blackleg development. The aim of this study was to identify which biotic and/or abiotic factors are most critical to the development of blackleg in the field. In Switzerland, the predominant species have belonged to Genus Dickeya as far back as 1992, which is why this study only investigates blackleg symptoms induced by Dickeya isolates. Seven field trials, in which inoculated tubers were planted, were conducted during a 3-year period and the number of blackleg-diseased plants was counted. Multiple regression analysis was used in order to determine the factors that had the greatest impact on two different variables: (i) periods between emergence of the plant and disease outbreak and (ii) overall blackleg incidence throughout the growing season. The results of this analysis have revealed that environmental factors, such as evapotranspiration and soil moisture, explain about half of the variability in the number of days before disease outbreak, and the total number of diseased plants is widely dependent upon cultivar susceptibility and isolate aggressiveness.
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References
Adams, S. S., & Stevenson, W. R. (1990). Water management, disease development, and potato production. American Potato Journal, 67(1), 3–11. doi:10.1007/bf02986908.
Agrometeo (2013). Prévision et gestion des risques pour l’agriculture, meteorology. Agroscope. http://www.agrometeo.ch/. Accessed 15 Nov 2013.
Aleck, J. R., & Harrison, M. D. (1978). Influence of inoculum density and environment on development of potato blackleg. American Potato Journal, 55(9), 479–494. doi:10.1007/bf02852154.
Allefs, J., VanDooijeweert, W., Prummel, W., Keizer, L. C. P., & Hoogendoorn, J. (1996). Components of partial resistance to potato blackleg caused by pectolytic Erwinia carotovora subsp atroseptica and E. chrysanthemi. Plant Pathology, 45(3), 486–496.
Bain, R. A., & Perombelon, M. C. M. (1990). Blackleg development and tuber yield in relation to numbers of Erwinia carotovora subsp atroseptica on seed potatoes. Plant Pathology, 39(1), 125–133. doi:10.1111/j.1365-3059.1990.tb02483.x.
Bain, R. A., Millard, P., & Perombelon, M. C. M. (1996). The resistance of potato plants to Erwinia carotovora subsp atroseptica in relation to their calcium and magnesium content. Potato Research, 39(2), 185–193. doi:10.1007/bf02358218.
Cazelles, O., & Schwaerzel, R. (1992). Enquête sur les bactérioses causées par Erwinia dans les cultures de plants de pommes de terre en Suisse romande. Revue Suisse d’Agriculture, 24(4), 215–218.
CIP. (2007). Procedures for standard evaluation trials of advanced potato clones. An International Cooperators’ Guide. Lima: International Potato Center (CIP).
Czajkowski, R., de Boer, W. J., Velvis, H., & van der Wolf, J. M. (2010). Systemic colonization of potato plants by a soilborne, green fluorescent protein-tagged strain of Dickeya sp biovar 3. Phytopathology, 100(2), 134–142.
Czajkowski, R., Perombelon, M. C. M., van Veen, J. A., & van der Wolf, J. M. (2011). Control of blackleg and tuber soft rot of potato caused by Pectobacterium and Dickeya species: a review. Plant Pathology, 60(6), 999–1013. doi:10.1111/j.1365-3059.2011.02470.x.
Czajkowski, R., de Boer, W. J., & van der Wolf, J. M. (2013). Chemical disinfectants can reduce potato blackleg caused by ‘Dickeya solani’. [Article]. European Journal of Plant Pathology, 136(2), 419–432. doi:10.1007/s10658-013-0177-8.
Dupuis, B., Schaerer, S., Gilliand, H., & Cazelles, O. (2010). The Dickeya and Pectobacterium situation in Switzerland. In Dickeya Workshop, Emmeloord, The Netherlands.
Gobat, J., Aragno, M., & Matthey, W. (2010). Le sol vivant: bases de pédologie, biologie des sols (Sciences de la terre ed.). PPUR Presses Polytechniques.
Graham, D. C., & Harper, P. C. (1966). Effect of inorganic fertilizers on the Incidence of potato blackleg disease. Potato Research, 9(3), 141–145.
Helias, V., Andrivon, D., & Jouan, B. (2000). Development of symptoms caused by Erwinia carotovora ssp atroseptica under field conditions and their effects on the yield of individual potato plants. Plant Pathology, 49(1), 23–32.
Laurila, J., Hannukkala, A., Nykyri, J., Pasanen, M., Helias, V., Garlant, L., et al. (2010). Symptoms and yield reduction caused by Dickeya spp. strains isolated from potato and river water in Finland. European Journal of Plant Pathology, 126(2), 249–262.
McGuire, R. G., & Kelman, A. (1984). Reduced severity of Erwinia soft rot in potato-tubers with increased calcium content. Phytopathology, 74(10), 1250–1256. doi:10.1094/Phyto-74-1250.
MeteoSwiss (2013). Portail des données de MétéoSuisse. MétéoSuisse. http://www.meteosuisse.admin.ch/web/fr/services/portail_des_donnees.html. Accessed 15 Nov 2013.
Pérombelon, M. C. M. (2002). Potato diseases caused by soft rot erwinias: an overview of pathogenesis. Plant Pathology, 51(1), 1–12. doi:10.1046/j.0032-0862.2001.Short title.doc.x.
Pérombelon, M. C. M., Lumb, V. M., Zutra, D., Hyman, L. J., & Burnett, E. M. (1989). Factors affecting potato blackleg development. In E. C. Tjamos & C. H. Beckman (Eds.), Vascular wilt diseases of plants (NATO ASI Series, Vol. 28, pp. 421–431). Berlin: Springer.
Prokkola, S. (1994). Effect of applying nitrogen-fertilizer to a potato seed crop on the susceptibility of the daughter plants to Erwinia carotovora subsp atroseptica. Potato Research, 37(1), 103–111. doi:10.1007/bf02358709.
Rouffiange, J., Gerardin, D., Kellenberger, I., Schaerer, S., & Dupuis, B. (2013). Sensibilité de la pomme de terre aux pourritures de tiges provoquées par Dickeya spp. Recherche Agronomique Suisse, 4(10), 424–431.
StatSoft (2013). Electronic statistics textbook, how to find relationship between variables, multiple regression. http://www.statsoft.com/Textbook/Multiple-Regression. Accessed 20 Nov 2013.
Tandogdu, Y., & Camgoz, O. (1999). An experimental approach for estimating evapotranspiration. CIM Bulletin, 92(1027), 55–60.
Toth, I. K., van der Wolf, J. M., Saddler, G., Lojkowska, E., Helias, V., Pirhonen, M., et al. (2011). Dickeya species: an emerging problem for potato production in Europe. [Review]. Plant Pathology, 60(3), 385–399. doi:10.1111/j.1365-3059.2011.02427.x.
Acknowledgments
Henri Gilliand, Isabelle Kellenberger, Gaétan Riot, Ruedi Schwaerzel, Maud Tallant, Etienne Thévoz, Jean-Marie Torche and Werner Wild are thanked for their technical support. David Gerardin, Solène Pierrucci and Jérémie Rouffiange are thanked for their contribution during their internship. BIOREBA AG and HAFL are thanked for their greatly appreciated partnership. Swisspatat, Swisssem and the Commission for Technology and Innovation CTI are gratefully acknowledged for their financial support.
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Gill, E.D., Schaerer, S. & Dupuis, B. Factors impacting blackleg development caused by Dickeya spp. in the field. Eur J Plant Pathol 140, 317–327 (2014). https://doi.org/10.1007/s10658-014-0465-y
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DOI: https://doi.org/10.1007/s10658-014-0465-y