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Gene cloning and expression analysis of AhR and CYP4 from Pinctada martensii after exposed to pyrene

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Abstract

Pyrene, a typical polycyclic aromatic hydrocarbon, is a common pollutant in the marine environment. Polycyclic aromatic hydrocarbons initiate cellular detoxification in an exposed organism via the activation of the aryl hydrocarbon receptor (AhR). Subsequent metabolism of these xenobiotics is mainly by the cytochrome P450 enzymes of the phase I detoxification system. Full-length complementary DNA sequences from the pearl oyster Pinctada martensii (pm) encoding AhR and cytochrome P4 were cloned. The P. martensii AhR complementary DNA sequence constitutes an open reading frame that encodes for 848 amino acids. Sequence analysis indicated PmAhR showed high similarity with its homologues of other bivalve species. The cytochrome P(CYP)4 complementary DNA sequence of P. martensii constitutes an open reading frame that encodes for 489 amino acids. Quantitative real-time analysis detected both PmAhR and PmCYP4 messenger RNA expressions in the mantle, gill, hepatapancreas and adductor muscle of P. martensii exposed to pyrene. The highest transcript-band intensities of PmAhR and PmCYP4 were observed in the gill. Temporal expression of PmAhR and PmCYP4 messenger RNAs induction was observed in gills and increased between 3 and 5 days post exposure; then returned to control level. These results suggest that messenger RNAs of PmAhR and PmCYP4 in pearl oysters might be useful parameters for monitoring marine environment pyrene pollution.

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Acknowledgments

This study was supported by National Natural Sciences Foundation of China (31160126). The authors would like to acknowledge all other members at Dr. Xiaoping Diao Laboratory for their help with sampling and taking care of the pearl oysters.

Conflict of interest

The authors declare that there are no conflicts of interest for their work.

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Authors and Affiliations

  1. College of Environment and Plant Protection, Hainan University, Haikou, 570228, China

    Junqiao Du & Fuqiang Wang

  2. College of Agriculture, Hainan University, Haikou, 570228, China

    Chenghong Liao, Hailong Zhou, Xiaoping Diao, Yuhu Li & Pengfei Zheng

  3. Haikou Key Laboratory of Environment Toxicology, Hainan University, Haikou, 570228, China

    Junqiao Du, Chenghong Liao, Hailong Zhou, Xiaoping Diao, Yuhu Li, Pengfei Zheng & Fuqiang Wang

  4. Key Laboratory of Tropical Biological Resources, Ministry of Education, Hainan University, Haikou, 570228, China

    Junqiao Du

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  1. Junqiao Du
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  4. Xiaoping Diao
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Correspondence to Xiaoping Diao.

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Du, J., Liao, C., Zhou, H. et al. Gene cloning and expression analysis of AhR and CYP4 from Pinctada martensii after exposed to pyrene. Ecotoxicology 24, 1574–1582 (2015). https://doi.org/10.1007/s10646-015-1424-x

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