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Estrogen alters the profile of the transcriptome in river snail Bellamya aeruginosa

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Abstract

We evaluated the transcriptome dynamics of the freshwater river snail Bellamya aeruginosa exposed to 17β-estradiol (E2) using the Roche/454 GS-FLX platform. In total, 41,869 unigenes, with an average length of 586 bp, representing 36,181 contigs and 5,688 singlets were obtained. Among them, 18.08, 36.85, and 25.47 % matched sequences in the GenBank non-redundant nucleic acid database, non-redundant protein database, and Swiss protein database, respectively. Annotation of the unigenes with gene ontology, and then mapping them to biological pathways, revealed large groups of genes related to growth, development, reproduction, signal transduction, and defense mechanisms. Significant differences were found in gene expression in both liver and testicular tissues between control and E2-exposed organisms. These changes in gene expression will help in understanding the molecular mechanisms of the response to physiological stress in the river snail exposed to estrogen, and will facilitate research into biological processes and underlying physiological adaptations to xenoestrogen exposure in gastropods.

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Abbreviations

E2:

17β-Estradiol

DMSO:

Dimethyl sulfoxide

RNA:

Ribonucleic acid

DNA:

Deoxyribonucleic acid

cDNA:

Complementary DNA

dNTP:

Deoxynucleotide triphosphate

DTT:

Dithiothreitol

AMV RT:

Avian myeloblastosis virus reverse transcriptase

T4 PNK:

T4 polynucleotide kinase

ATP:

Adenosine triphosphate

RPKM:

Reads per kilobase transcript per million reads

FDR:

False discovery rate

GO:

Gene ontology

KEGG:

Kyoto encyclopedia of genes and genomes

ORF:

Open reading frames

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Acknowledgments

Financial supports by National Natural Science Foundation of China (41330637 & 21177117) are gratefully acknowledged.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environment Sciences, No. 8, Da-Yang-Fang, An-Wai-Bei-Yuan Rd., Chao-yang District, Beijing, 100012, China

    Kun Lei, Ruizhi Liu & Li-hui An

  2. Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100029, China

    Ying-feng Luo

  3. Department of Biological Sciences, Environmental and Molecular Toxicology Program, North Carolina State University, Raleigh, NC, 27606, USA

    Gerald A. LeBlanc

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  1. Kun Lei
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  2. Ruizhi Liu
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  3. Li-hui An
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  4. Ying-feng Luo
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  5. Gerald A. LeBlanc
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Correspondence to Li-hui An.

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Lei, K., Liu, R., An, Lh. et al. Estrogen alters the profile of the transcriptome in river snail Bellamya aeruginosa . Ecotoxicology 24, 330–338 (2015). https://doi.org/10.1007/s10646-014-1381-9

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