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Use of an alternating current amplifier when recording the ERG c-wave to evaluate the function of retinal pigment epithelial cells in rats

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Abstract

Purpose

We studied the conditions under which c-waves of the electroretinogram (ERG), that represent retinal pigment epithelium (RPE) function, were detectable using an alternating current (AC) amplifier and whether the c-wave recorded using an AC amplifier was useful for evaluating RPE function.

Methods

We recorded ERG responses in rats to 5 s stimuli under the conditions in which the low-cut frequency and the stimulus luminance were varied. In addition, changes in ERGs were studied after intravenous injection of sodium iodate (SI) to induce RPE degeneration.

Results

The c-wave was detected clearly when the frequency of the low-cut filter was set at 0.01 Hz and light stimulus luminances were ≥ − 1.0 log cd/m2. The c-wave was attenuated earlier than other waves (e.g., a-wave and b-wave) after SI administration.

Conclusions

The c-wave was easily detectable using an AC amplifier with the low-cut filter set at 0.01 Hz. Using the AC amplifier may allow easier c-wave recording, compared with the conventional use of a direct current (DC) amplifier, and could be useful for evaluating RPE function.

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Acknowledgements

The authors thank Mr. Hidetaka Kudo of Mayo Corporation for technical assistance.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Preclinical Research Unit, Drug Research Division, Sumitomo Pharma Co. Ltd, 3-1-98 Kasugade-naka, Konohana-ku, Osaka, 554-0022, Japan

    Naohisa Umeya, Izuru Miyawaki & Hiroshi Inada

Authors
  1. Naohisa Umeya
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  2. Izuru Miyawaki
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  3. Hiroshi Inada
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Contributions

Naohisa Umeya designed the study, collected and analyzed data, and wrote the initial draft of the manuscript. Izuru Miyawaki and Hiroshi Inada contributed to the interpretation of data and assisted in the preparation of the manuscript. All authors approved the final version of the manuscript and agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

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Correspondence to Naohisa Umeya.

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All authors certify that they have no affiliations with or involvement in any organization or entity with any financial or non-financial interest in the subject matter or materials discussed in this manuscript.

Ethics approval (include appropriate approvals or waivers)

All procedures in this study were approved by the Institutional Animal Care and Use Committee of Drug Research Division, Sumitomo Pharma Co., Ltd.

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This article does not contain any studies with humans performed by any of the authors.

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This article does not contain any studies with humans performed by any of the authors.

Statement on the welfare of animals

All procedures in this study were approved by the Institutional Animal Care and Use Committee of Drug Research Division, Sumitomo Pharma Co., Ltd.

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Umeya, N., Miyawaki, I. & Inada, H. Use of an alternating current amplifier when recording the ERG c-wave to evaluate the function of retinal pigment epithelial cells in rats. Doc Ophthalmol 145, 147–155 (2022). https://doi.org/10.1007/s10633-022-09890-y

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  • DOI: https://doi.org/10.1007/s10633-022-09890-y

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