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Serum Cyclophilin A Correlates with Increased Tissue MMP-9 in Patients with Ulcerative Colitis, but Not with Crohn’s Disease

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Abstract

Background

Cyclophilin A (CyPA) is an immunomodulatory protein, high expression of which correlates with poor outcome of patients with inflammatory diseases. However, its role in inflammatory bowel disease (IBD) has not been studied.

Aim

This study analyzes the correlation between cyclophilin A, matrix metalloproteinase (MMP)-9, and tissue inhibitor of MMP (TIMP)/MMP-9 complexes in the inflamed and non-inflamed colon mucosa of UC and CD patients.

Methods

Serum and biopsy specimens from inflamed and non-inflamed colonic mucosa of 38 patients with IBD (19 with UC and 19 with CD) and 16 controls were included in our study. We measured serum and tissue level of CyPA, and tissue level of TNF-α, MMP-9, TIMP-1/MMP-9, and TIMP-2/MMP-9 using ELISA method.

Results

Our results indicated that serum, but not tissue CyPA is increased in UC, rather than in CD patients, compared to the control. The increase correlated with higher tissue concentration of MMP-9 and TNF-α, especially in the UC group. Moreover, we observed significantly higher level of TIMP-1/MMP-9 in UC and CD group, which overlapped with the change in MMP-9. There was no change in TIMP-2/MMP-9 in the analyzed groups.

Conclusion

The current study suggests that serum CyPA may be an independent additional marker of IBD, especially of UC. Higher CyPA level may be followed by increased MMP-9 in those patients. However, further studies are necessary to verify the role of CyPA in IBD development.

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Acknowledgments

Supported by the grants from the Medical University of Lodz (#503/1-156-04/503-01 to JF). Aleksandra Piechota-Polanczyk is currently working at the Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland. Faculty of Biochemistry, Biophysics and Biotechnology of Jagiellonian University is a partner of the Leading National Research Center (KNOW) supported by the Ministry of Science and Higher Education.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, Lodz, Poland

    Aleksandra Piechota-Polanczyk, Marcin Włodarczyk, Aleksandra Sobolewska-Włodarczyk, Mateusz Jonakowski, Andrzej Pilarczyk & Jakub Fichna

  2. Department of Gastroenterology, Faculty of Military Medicine, Medical University of Lodz, Lodz, Poland

    Aleksandra Sobolewska-Włodarczyk, Krystyna Stec-Michalska & Maria Wiśniewska-Jarosińska

  3. Department of General and Colorectal Surgery, Faculty of Military Medicine, Medical University of Lodz, Lodz, Poland

    Marcin Włodarczyk

  4. Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Kraków, Poland

    Aleksandra Piechota-Polanczyk

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  1. Aleksandra Piechota-Polanczyk
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  2. Marcin Włodarczyk
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Correspondence to Aleksandra Piechota-Polanczyk.

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Piechota-Polanczyk, A., Włodarczyk, M., Sobolewska-Włodarczyk, A. et al. Serum Cyclophilin A Correlates with Increased Tissue MMP-9 in Patients with Ulcerative Colitis, but Not with Crohn’s Disease. Dig Dis Sci 62, 1511–1517 (2017). https://doi.org/10.1007/s10620-017-4568-0

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  • DOI: https://doi.org/10.1007/s10620-017-4568-0

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