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Biotransformation of Dehydroepiandrosterone by Cell Suspension Culture of Codiaeum variegatum

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Dehydroepiandrosterone (1) was incubated with a cell suspension culture of Codiaeum variegatum for biotransformational studies. After 10 days of incubation, four metabolites, 5α-androstane-3,17-dione (2), 5β-androstane-3,17-dione (3), androst-4-ene-3,17-dione (4), and 17β-hydroxyandrost-4-en-3-one (5), were obtained. The structures of these biotransformed products were elucidated by spectroscopic methods. Biotransformation using a cell suspension culture of Codiaeum variegatum provides an effective and convenient method to oxidize the 3β-hydroxy group in 1.

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Acknowledgment

One of us (Azizuddin) acknowledges the enabling role of the Federal Urdu University of Arts, Science & Technology, Gulshan-e-Iqbal, Karachi, Pakistan, and appreciate its financial support through the “Mini Research Project.”

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Authors and Affiliations

  1. H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, 75270, Karachi, Pakistan

    Saifullah, Saifullah Khan, Kashif Ali & Muhammad Iqbal Choudhary

  2. Department of Chemistry, Federal Urdu University of Arts, Science & Technology, Gulshan-e-Iqbal Campus, 75300, Karachi, Pakistan

    Azizuddin

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  1. Saifullah
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  2. Azizuddin
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  3. Saifullah Khan
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  4. Kashif Ali
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  5. Muhammad Iqbal Choudhary
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Correspondence to Azizuddin.

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Published in Khimiya Prirodnykh Soedinenii, No. 4, July–August, 2014, pp. 580–582.

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Saifullah, Azizuddin, Khan, S. et al. Biotransformation of Dehydroepiandrosterone by Cell Suspension Culture of Codiaeum variegatum . Chem Nat Compd 50, 669–672 (2014). https://doi.org/10.1007/s10600-014-1049-y

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  • DOI: https://doi.org/10.1007/s10600-014-1049-y

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