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Spatial genetic structure of a keystone long-lived semiarid shrub: historical effects prevail but do not cancel the impact of recent severe habitat loss on genetic diversity

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Abstract

Land-use conversion and habitat loss and degradation are among the factors affecting populations and species genetic integrity. Understanding how these factors govern the genetic structure of threatened plant populations is essential to design efficient conservation strategies. Here we analyze how environmental correlates, geographic location and anthropogenic disturbance (at local, landscape and regional scales) relate to population differentiation and genetic diversity of a keystone semiarid long-lived shrub, Ziziphus lotus. The European range of this species is restricted mainly to the southeast of the Iberian Peninsula (> 90%) but its area has shrunk considerably as a consequence of intensive agriculture and urban expansion since the 1950s. We characterize its genetic diversity and structure across the Iberian Peninsula with microsatellite markers, considering 21 populations. All populations showed high and relatively similar values of genetic diversity (mean/pop: Ho = 0.54; He = 0.55; AR = 3.59). Three main genetic clusters were defined by clustering analyses corresponding largely to west, central (core) and east of its distribution. Molecular variance analysis revealed that 87.3% of genetic variation was grouped within populations, 5% among populations and 7% between regions. Geographic location (37.88%) has stronger correlation with population genetic differences than landscape characteristics (5.82%). Natural habitat cover at the landscape level was the only environmental variable associated with FST population differences. The detected population genetic patterns are probably related primarily to historical gene flow and secondarily to intensive land-use change of this area in the last 60 years. Our results suggest that, although the long-lived and resprouting strategy of the species is buffering the erosion of genetic diversity at population and regional level, there are already genetic consequences of landscape degradation, which could be aggravated in the future.

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Acknowledgements

Thanks J.M. Bastida for his help throughout the study; J. Isla, J.L. González-Rebollar and A.B. Robles for their advice and assistance during field samplings; CICT of Universidad de Jaén for fragment sequencing work; Junta de Andalucía and M.A. Carrión (Dirección General de Medio Ambiente del Gobierno de Murcia) for facilitating permits for Almería and Murcia field samplings, respectively; and two anonymous reviewers for useful comments and suggestions that improved the manuscript. This work was funded by the RNM-766 project of Junta de Andalucía and by funds from Fondo Europeo de Desarrollo Regional (FEDER). A. González-Robles was supported by Universidad de Jaén predoctoral fellowship, A.J. Manzaneda by a Ramón y Cajal contract (RYC-2010-06237) and C. García by Fundação para a Ciência e a Tecnologia (FCT) through the Independent Fellowship IF-Investigador program (IF/01375/2012).

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  1. Departamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén, 23071, Jaén, Spain

    Ana González-Robles, Antonio J. Manzaneda, Teresa Salido, Ana M. Fernández-Ocaña & Pedro J. Rey

  2. Estación Experimental de Zonas Áridas, EEZA-CSIC, 04120, Almería, Spain

    Francisco Valera

  3. Department of Evolution, Ecology and Behaviour, Institute of Integrative Biology, University of Liverpool, Leahurst Campus, Neston, CH64 7TE, UK

    Cristina García

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  1. Ana González-Robles
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González-Robles, A., Manzaneda, A.J., Salido, T. et al. Spatial genetic structure of a keystone long-lived semiarid shrub: historical effects prevail but do not cancel the impact of recent severe habitat loss on genetic diversity. Conserv Genet 21, 853–867 (2020). https://doi.org/10.1007/s10592-020-01291-5

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