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Conservation genetics of redside dace (Clinostomus elongatus): phylogeography and contemporary spatial structure

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Abstract

Redside dace Clinostomus elongatus (Teleostei: Cyprinidae) is a species of conservation concern that is declining throughout its range as a result of habitat fragmentation, degradation and loss. We characterized the genetic structure and diversity of redside dace populations across the species range using mitochondrial and microsatellite data to inform conservation efforts and assess how historical and recent events have shaped genetic structure and diversity within and among populations. Phylogeographic structure among 28 redside dace populations throughout southern Ontario (Canada) and the United States was assessed by sequence analysis of the mitochondrial cytochrome b and ATPase 6 and 8 genes. Populations were also genotyped using ten microsatellite loci to examine genetic diversity within and among populations as well as contemporary spatial structuring. Mitochondrial DNA sequence data revealed three geographically distinct lineages, which were highly concordant with groupings identified by microsatellite analysis. The combined genetic data refute published glacial refugia hypotheses of a single Mississippian refugium or of two lineages associated with Mississippian and Atlantic refugia. Secondary contact between the two eastern groups was documented in the Allegheny River drainage and tributaries to Lake Ontario. With the exception of several allopatric populations within the Allegheny watershed, high genetic structuring among populations suggests their isolation, indicating that recovery efforts should be population-based.

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Acknowledgements

Funding for this project was provided by the Ontario Ministry of Natural Resources and Forestry (OMNRF) and Fisheries and Oceans Canada. Samples were generously provided by Jeff Anderson (Conservation Halton), Douglas Carlson (New York State Department of Environmental Conservation), Jon Clayton (Credit Valley Conservation), Konrad Schmidt and Jenny Kruckenberg (North American Native Fishes Association), Holly Jennings and John Pagel (USDA Forest Service, Michigan), Brant Fisher (Indiana Department of Natural Resources), Brian Zimmerman (Ohio State University), David Thorne and Isaac Gibson (West Virginia Division of Natural Resources), John Lyons (Wisconsin Department of Natural Resources), Nate Tessler (EnviroScience, Inc.), David Miko (Pennsylvania Fish and Boat Commission), and Matthew Thomas (Kentucky Department of Fish and Wildlife Resources). Aaron Clauser (Clauser Environmental), Wayne Starnes (North Carolina State Museum of Natural Sciences), Aaron Snell (Streamside Ecological Services, Inc), and Douglas Fischer (Pennsylvania Fish and Boat Commission) also provided valuable advice and feedback. Lab work assistance was provided by Caleigh Smith, Kristyne Smith, and Anne Kidd. Alan Dextrase (OMNRF) and Joanna Freeland (Trent University) and two anonymous reviewers provided constructive comments on earlier drafts of the manuscript

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  1. Environmental and Life Sciences Graduate Program, Trent University, 2140 East Bank Drive, Peterborough, ON, K9J 7P8, Canada

    Natasha R. Serrao

  2. Aquatic Research and Monitoring Section, Ontario Ministry of Natural Resources and Forestry, Trent University, 2140 East Bank Drive, Peterborough, ON, K9J 7P8, Canada

    Natasha R. Serrao, Scott M. Reid & Chris C. Wilson

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  1. Natasha R. Serrao
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Serrao, N.R., Reid, S.M. & Wilson, C.C. Conservation genetics of redside dace (Clinostomus elongatus): phylogeography and contemporary spatial structure. Conserv Genet 19, 409–424 (2018). https://doi.org/10.1007/s10592-017-1012-0

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