Abstract
Interneurons in the olfactory bulb (OB) are generated from neuronal precursor cells migrating from anterior subventricular zone (SVZa) not only in the developing embryo but also throughout the postnatal life of mammals. In the present study, we established an in vivo electroporation assay to label SVZa cells of rat both at embryonic and postnatal ages, and traced SVZa progenitors and followed their migration pathway and differentiation. We found that labeled cells displayed high motility. Interestingly, the postnatal cells migrated faster than the embryonic cells after applying this assay at different ages of brain development. Furthermore, based on brain slice culture and time-lapse imaging, we analyzed the detail migratory properties of these labeled precursor neurons. Finally, tissue transplantation experiments revealed that cells already migrated in subependymal zone of OB were transplanted back into rostral migratory stream (RMS), and these cells could still migrate out tangentially along RMS to OB. Taken together, these findings provide an in vivo labeling assay to follow and trace migrating cells in the RMS, their maturation and integration into OB neuron network, and unrecognized phenomena that postnatal SVZa progenitor cells with higher motility than embryonic cells, and their migration was affected by extrinsic environments.
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Acknowledgements
We acknowledge that this study was funded by the several grants as follows: Zhejiang Provincial Natural Science Foundation of China under Grant (LY15C090006, Q17H090034, R18C090008), Science and Technology Planning Project of Zhejiang Province (2017C33197), National Natural Science Foundation (3167107, 181371350), and the Outstanding Leaders Training Program of Pudong Health Bureau of Shanghai (NO. PWR12015-06).
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ZHH and YW planned the study, wrote the main manuscript, and performed the main experiments. YWX, ZYL, JD, and TTW performed staining experiments. SXH, YC, and BYC were responsible for the cultivation of experiments, providing general coordination of the study, and revised the manuscript. All authors have read and approved the final manuscript.
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Xie, Yw., Li, Zy., Du, J. et al. Visualization of Rostral Migratory Stream in the Developing Rat Brain by In Vivo Electroporation. Cell Mol Neurobiol 38, 1067–1079 (2018). https://doi.org/10.1007/s10571-018-0577-6
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DOI: https://doi.org/10.1007/s10571-018-0577-6