Abstract
In the current study, we aimed to develop a drug-delivery wound dressing by incorporation of mequinol into the matrix of electrospun chitosan/carboxymethyl cellulose (CMC)-based scaffolds. Mequinol was added to the chitosan/CMC solution at three different concentrations of 0.3% w/w, 0.6% w/w, and 0.9% w/w and then electrospun. The physicochemical and biological properties of electrospun scaffolds were studied. Cell culture studies revealed that the dressings containing 0.3% drug had the highest cell viability and protection against oxidative stress. Wound healing assay and in vitro characterization experiments were performed on this formulation. In vitro studies showed that the incorporation of mequinol into the matrix of electrospun scaffolds significantly improved their anti-inflammatory and antioxidant activities. Wound healing assay showed that chitosan/CMC/0.3% mequinol wound dressings had significantly higher rate of wound size reduction, epithelial thickness, and tissue repair compared with drug-free scaffolds and negative control group. Gene expression analysis showed that the wounds treated with chitosan/CMC/0.3% mequinol scaffolds decreased the tissue expression level of glutathione peroxidase, TNF-a, and IL-1β genes. This study suggests potential use of the proposed wound dressings for the treatment of diabetic wounds in the clinic.
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The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgments
This research was funded by Princess Nourah bint Abdulrahman University Researchers Supporting Project number (PNURSP2022R145), Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia. This study is part of an award-winning large scale research project financed by Taif University Researchers Supporting Project Number (TURSP-2020/117).
Funding
This research was funded by Princess Nourah bint Abdulrahman University Researchers Supporting Project number (PNURSP2022R145), Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia. This study is part of an award-winning large scale research project financed by Taif University Researchers Supporting Project Number (TURSP-2020/117).
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WKA, SME, KAI conceptualized and designed the whole study. ME, KAI, HSAG, SM, and HSH. Ridha performed the experiments and analyzed the data. MSM, LT, TM, YFM prepared the first darft of the manuscript, analyzed data, and performed some experiments.
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Abdelbasset, W.K., Elkholi, S.M., Ismail, K.A. et al. Mequinol-loaded carboxymethyl cellulose/chitosan electrospun wound dressing as a potential candidate to treat diabetic wounds. Cellulose 29, 7863–7881 (2022). https://doi.org/10.1007/s10570-022-04753-w
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DOI: https://doi.org/10.1007/s10570-022-04753-w