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Parenchyma cell wall structure in twining stem of Dioscorea balcanica

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Abstract

Anatomical adaptation of liana plants includes structural changes in cell walls of different tissues: fibers, vessel elements and tracheids. However, the contribution of parenchyma cells to stem twining in liana plants is mostly unknown. The aim of this investigation is to determine changes in stem parenchyma cell walls that are correlated with the twinning process in liana plants. Parenchyma cell wall structure was studied on the stem cross sections of straight and twisted internodes of monocotyledonous liana Dioscorea balcanica, by different microscopy techniques: light microscopy, scanning electron microscopy, fluorescence detected linear dichroism microscopy and Fourier transform infrared microspectrometry. In addition, chemical analysis of the entire stem internodes was performed using photometric and chromatographic methods. Parenchyma cell walls of twisted D. balcanica internodes are characterized by: lower amounts of cellulose (obtained by FTIR microspectrometry) with different cellulose microfibril orientation (shown by Scanning electron microscopy), but no changes in “cellulose fibril order” (obtained by Differential polarization laser scanning microscopy); lower amounts of xyloglucan, higher amounts of xylan, higher amounts of lignin with modified organization—less condensed lignin (obtained by FTIR microspectrometry). At the same time, chemical analysis of the entire internodes did not show significant differences in lignin content and cell wall bound phenols related to stem twining, except for the presence of diferulate cross-links exclusively in twisted internodes. Our results indicate that adaptations to mechanical strain in D. balcanica stems involve modifications in parenchyma cell wall structure and chemistry, which provide decreased stiffness, higher strength and increased elasticity of twisted internodes.

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  • 23 February 2018

    In the original publication of the article, one of the project numbers was omitted in the Acknowledgments. The correct version is provided below.

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Acknowledgments

This work was supported by: the Grants 173017 and 173015 of the Ministry of education and science of the Republic of Serbia, Project Algamic (CZ 1.05/2.1.00/19.0392) of Czech Republic. The authors wish to thank Prof. Ljubinka Ćulafić for providing D. balcanica photographs.

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Authors and Affiliations

  1. Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, Belgrade, 11000, Serbia

    Jasna Simonović Radosavljević, Jelena Bogdanović Pristov, Aleksandra Lj. Mitrović, Vuk Maksimović, Dragosav Mutavdžić, Marina Vuković & Ksenija Radotić

  2. Institute of Biophysics, Biological Research Center, Szeged, 6701, Hungary

    Gabor Steinbach

  3. Institute of Microbiology, CAS, Centrum Algatech, 379 01, Třeboň, Czech Republic

    Gabor Steinbach

  4. INRA, Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech, ERL3559 CNRS, Saclay Plant Sciences, 78026, Versailles, France

    Gregory Mouille

  5. Department of Chemistry, Institute of Chemistry, Technology and Metallurgy, Njegoševa 12, POB 815, Belgrade, Serbia

    Srđan Tufegdžić

  6. Institute of Botany and Botanical Garden, Jevremovac“, Faculty of Biology, University of Belgrade, Takovska 43, Belgrade, Serbia

    Dušica Janošević

  7. Institute of Plant Biology, Biological Research Center, Szeged, 6701, Hungary

    Győző Garab

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  1. Jasna Simonović Radosavljević
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  2. Jelena Bogdanović Pristov
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Correspondence to Ksenija Radotić.

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A correction to this article is available online at https://doi.org/10.1007/s10570-018-1706-6.

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Simonović Radosavljević, J., Bogdanović Pristov, J., Lj. Mitrović, A. et al. Parenchyma cell wall structure in twining stem of Dioscorea balcanica . Cellulose 24, 4653–4669 (2017). https://doi.org/10.1007/s10570-017-1460-1

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