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A Positive Effect of Magnetic Field on the Catalytic Activity of Immobilized L-Asparaginase: Evaluation of its Feasibility

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Abstract

Enzyme immobilization is an attractive strategy to improve enzyme stability, however, the activity significantly reduces after immobilization. To solve this issue, we designed a novel magnetic carrier that both enhanced enzyme activity and improved its stability. For this purpose, the magnetic nanoparticles were synthesized and l-asparaginase was immobilized physically. All materials were structurally and morphologically characterized. Besides, the biochemical properties of the immobilized enzyme were investigated and compared with the free one. Moreover, the activity of the immobilized enzyme was investigated under a weak magnetic field. The optimum pH and optimum temperature of the free and immobilized enzyme were found to be 8.5 and 45 °C, 7.5 and 40 °C, respectively. Moreover, even after 10 cycles of use, the immobilized enzyme retained 54% of its initial activity. Km for free and the immobilized enzyme was found to be 10.37 ± 0.5, and 7.06 ± 2.99 mM, respectively, and Vmax was found to be 138.88 ± 2.64, and 121.95 ± 1.07 µmol/min, respectively. Most importantly, the activity increased approximately 3.2-fold and 4.3-fold at 10 Hz and 20 mT, respectively. Overall, the results suggested that, if the activity of the immobilized enzyme is very low, applying a weak magnetic field may be necessary to enhance the enzyme reaction.

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Acknowledgements

The authors are grateful to the Scientific Research Projects Unit of Inönü University (project number FYL-2019-1865) and to the National Boron Research Institute to recognize the financial support. This study was derived from the master thesis presented by Gamze Dik (2021-685201).

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Authors and Affiliations

  1. Biochemistry and Biomaterials Research Laboratory, Department of Chemistry, Faculty of Arts and Science, Inönü University, 44280, Malatya, Turkey

    Gamze Dik, Ahmet Ulu & Burhan Ateş

  2. Department of Physics, Faculty of Arts and Science, Inönü University, 44280, Malatya, Turkey

    Orhan Orçun Inan & Selçuk Atalay

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  1. Gamze Dik
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  2. Ahmet Ulu
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Contributions

GD: Methodology, Formal Analysis, and Writing-Original Draft Preparation. AU: Conceptualization, Methodology, Formal Analysis, Writing-Original Draft Preparation, and Writing-Reviewing and Editing. OOI: Investigation, Methodology, Formal Analysis. SA: Conceptualization, Investigation, Methodology, Formal Analysis, Resources, Supervision, Writing-Original Draft Preparation, and Writing-Reviewing and Editing. BA: Conceptualization, Investigation, Methodology, Formal Analysis, Resources, Supervision, Writing-Original Draft Preparation, and Writing-Reviewing and Editing.

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Correspondence to Ahmet Ulu or Burhan Ateş.

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Dik, G., Ulu, A., Inan, O.O. et al. A Positive Effect of Magnetic Field on the Catalytic Activity of Immobilized L-Asparaginase: Evaluation of its Feasibility. Catal Lett 153, 1250–1264 (2023). https://doi.org/10.1007/s10562-022-04075-3

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  • DOI: https://doi.org/10.1007/s10562-022-04075-3

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