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Insulin-like growth factor binding protein 5 accelerate the senescence of periodontal ligament stem cells

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Abstract

Evidences have showed stem cell mediated tissue regeneration is a promising method for the treatment of periodontitis. Insulin-like growth factor binding proteins-5 (IGFBP5) is a member of the insulin growth factor (IGFs) family and plays a regulatory role in cell proliferation and differentiation. Our previous study showed that IGFBP5 can promote osteogenic differentiation of periodontal ligament stem cells (PDLSCs) and enhance periodontal tissue regeneration mediated by PDLSCs. However, the function of IGFBP5 in the process of PDLSCs senescence remains unclear. The present study showed IGFBP5 mRNA level was highly expressed in passage-induced aged PDLSCs cells. IGFBP5 knockdown decreased the ratio of senescence associated β-galactosidase (SA-β-Gal) positive cells, enhanced the activity of TERT, and down-regulated the expression levels of P16, P21, P53 mRNA and protein. Overexpression of IGFBP5 increased the ratio of SA-β-Gal positive staining PDLSCs, decreased the activity of telomerase TERT, and up-regulated the expression levels of P16, P21, P53 mRNA and protein related to PDLSCs senescence. In conclusion, IGFBP5 can accelerate the senescence of PDLSCs, indicating the potential target for maintaining the "young state" of stem cells.

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All the datasets are available from the corresponding author on reasonable request.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (81670948 to R.D.).

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Author notes

  1. Le Li and Haiyan Wang have been contributed equally to this work.

Authors and Affiliations

  1. Department of Stomatology, Tsinghua University Hospital, Beijing, 100084, China

    Le Li

  2. Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Tian Tan Xi Li No.4, Beijing, 100050, People’s Republic of China

    Haiyan Wang, Dianqin Yu, Yantong Wang, Yingchu Zhao, Haoqing Yang, Yangyang Cao & Yu Cao

  3. Department of General Dentistry, Capital Medical University School of Stomatology, Tian Tan Xi Li No.4, Beijing, 100050, People’s Republic of China

    Yu Cao

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  1. Le Li
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  2. Haiyan Wang
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Correspondence to Yu Cao.

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Li, L., Wang, H., Yu, D. et al. Insulin-like growth factor binding protein 5 accelerate the senescence of periodontal ligament stem cells. Cell Tissue Bank 24, 231–239 (2023). https://doi.org/10.1007/s10561-022-10030-8

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