Skip to main content
SpringerLink
Log in

Resveratrol and Puerarin loaded polymeric nanoparticles to enhance the chemotherapeutic efficacy in spinal cord injury

  • Published:
Biomedical Microdevices Aims and scope Submit manuscript

Abstract

Spinal cord injury includes inflammation and apoptosis of neurons, which is difficult to cure by systemic drug administration. Administration of natural active compounds (resveratrol and also Puerarin) by advance drug delivery technology improves the patient’s conditions. Oil-in-water emulsion method was utilized to prepare resveratrol as well as puerarin loaded PLGA nanoparticles. The nanoparticles were subjected to mean zeta potential, mean particle size, encapsulation efficiency as well as in vitro drug release studies. The biochemical parameters i.e. malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), advanced oxidation products (AOPP), catalase (CAT) and nitrite/nitrate levels were tested for the loaded nanoparticles. Reperfusion injury induced rats treated with 10 mg/kg resveratrol and puerarin loaded nanoparticles protects spine from ischemia injury and supports biological parameters. The mean particle size varies from 238 nm to 274 nm and also particle size distribution was mono-dispersed (0.239 to 0.318). Zeta potential value of nanoparticles was observed to be −12.6 ± 2.1 mV. Optimized nanoparticles reveals 72% -79% of drug release over 36 h by diffusion mechanism. Significantly, lowers the levels of plasma nitrite/nitrate level as well as phosphorylation of p38MAPK pathways in reperfusion injury induced rats. The resveratrol and puerarin loaded nanoparticles decreases free radicals produced by reperfusion injury induced rats, as well as decrease of oxidative stress because of IRI. Resveratrol and puerarin loaded nanoparticles decreases GSH, SOD and CAT antioxidant level, which helps in overall health improvement of patients.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • M.D. Baumann, C.E. Kang, C.H. Tator, M.S. Shoichet, Biomaterials 31(30), 7631–7639 (2010)

    Article  Google Scholar 

  • P. Bremner, D. Rivera, M.A. Calzado, C. Obon, C. Inocencio, C. Beckwith, B.L. Fiebich, E. Munoz, M. Heinrich, J. Ethnopharmacol. 124(2), 295–305 (2009)

    Article  Google Scholar 

  • A. Conti, M. Miscusi, S. Cardali, A. Germano, H. Suzuki, S. Cuzzocrea, F. Tomasello, Brain Res. Rev. 54(1), 205–218 (2007)

    Article  Google Scholar 

  • C.L. Crowley-Weber, K. Dvorakova, C. Crowley, H. Bernstein, C. Bernstein, H. Garewal, C.M. Payne, Chem. Biol. Interact. 145(1), 53–66 (2003)

    Article  Google Scholar 

  • T.C. Chou, Cancer Res. 70(2), 440–446 (2010)

    Article  Google Scholar 

  • C. Mohanty, S. Acharya, A.K. Mohanty, F. Dilnawaz, S.K. Sahoo, Nanomedicine (Lond) 5(3), 433–449 (2010a)

    Article  Google Scholar 

  • S. Bhaumik, R. Anjum, N. Rangaraj, B.V. Pardhasaradhi, A. Khar, FEBS Lett. 456(2), 311–314 (1999)

    Article  Google Scholar 

  • H. Terada, T. Kazui, M. Takinami, K. Yamashita, N. Washiyama, B.A. Muhammad, J. Thorac. Cardiovasc. Surg. 122(5), 979–985 (2001)

    Article  Google Scholar 

  • L. Frémont, Life Sci. 66(1), 663–673 (2000)

    Article  Google Scholar 

  • Y. Song, J. Liu, F. Zhang, J. Zhang, T. Shi, Z. Zeng, Life Sci. 92(24–26), 1215–1221 (2013)

    Article  Google Scholar 

  • H.P. Misra, I. Fridovich, J. Biol. Chem. 247(10), 3170–3175 (1972)

    Google Scholar 

  • I. Pekarkova, S. Parara, V. Holecek, P. Stopka, L. Trefil, J. Racek, R. Rokyta, Physiol. Res. 50(6), 595–602 (2001)

    Google Scholar 

  • S. Takahara, H.B. Hamilton, J.V. Neel, T.Y. Kobara, Y. Ogura, E.T. Nishimura, J. Clin. Invest. 39(2), 610–619 (1960)

    Article  Google Scholar 

  • A.K. Mohanty, G.P. Mohanta, Int J Pharm Pharm Sci 6(9), 328–332 (2014)

    Google Scholar 

  • G.M. Cruise, D.S. Scharp, J.A. Hubbell, Biomaterials 19(14), 1287–1294 (1998)

    Article  Google Scholar 

  • H. Ohkawa, N. Ohishi, K. Yagi, Anal. Biochem. 95(2), 351–358 (1979)

    Article  Google Scholar 

  • A.K. Mohanty, F. Dilnawaz, C. Mohanty, S.K. Sahoo, Drug Deliv. 17(5), 330–342 (2010b)

    Article  Google Scholar 

  • S. Muthukkumar, T. Ramesh, S. Bondada, Transplantation 60(3), 264–270 (1995)

    Article  Google Scholar 

  • M. Dunne, I. Corrigan, Z. Ramtoola, Biomaterials 21(16), 1659–1668 (2000)

    Article  Google Scholar 

  • S.K. Sahoo, W. Ma, V. Labhasetwar, Int. J. Cancer 112(2), 335–340 (2004)

    Article  Google Scholar 

  • G. Lafci, H.S. Gedik, K. Korkmaz, H. Erdem, O.F. Cicek, O.A. Nacar, L. Yildirim, E. Kaya, H. Ankarali, J. Cardiothorac. Surg. 8(1), 64–69 (2013)

    Article  Google Scholar 

  • V.P. Lemmon, A.R. Ferguson, P.G. Popovich, X.M. Xu, D.M. Snow, M. Igarashi, C.E. Beattie, J.L. Bixby, J. Neurotrauma 31(15), 1354–1361 (2014)

    Article  Google Scholar 

  • G.L. Ellman, Arch. Biochem. Biophys. 82(1), 70–77 (1959)

    Article  Google Scholar 

  • G. Sa, T. Das, Cell Div 3(1), 3–14 (2008)

    Article  Google Scholar 

  • M.E. Mackay, A. Tuteja, P.M. Duxbury, C.J. Hawker, B. Van Horn, Z. Guan, G. Chen, R.S. Krishnan, Science 311(5768), 1740–1743 (2006)

    Article  Google Scholar 

  • D.M. Maggio, K. Chatzipanteli, N. Masters, S.P. Patel, W.D. Dietrich, D.D. Pearse, J. Neurotrauma 29(12), 2244–2249 (2012)

    Article  Google Scholar 

  • A.M. Sgaravatti, A.S. Magnusson, A.S. Oliveira, C.P. Mescka, F. Zanin, M.B. Sgarbi, C.D. Pederzolli, A.T. Wyse, Wannmacher, M. Wajner, C.S. Dutra-Filho, Metab. Brain Dis. 24(2), 271–282 (2008)

    Article  Google Scholar 

Download references

Acknowledgments

Qimin Song is extremely thankful to Department of Neurosurgery, Linyi central hospital, China for providing desired facility to carry out the research activity and other relevant supports.

Funding

This study was supported by funds from the Health Department of Shandong Province of China (grant nos. 2011QW008 and 2015WS0375).

Author information

Authors and Affiliations

  1. Department of Neurosurgery, Linyi central hospital, Yishui county, Linyi, 276400, Shandong province, China

    Wei Chen

  2. Department of pathology, Qingdao Hiser Hospital, Qingdao, 266033, Shandong, China

    Zongqin Zhao

  3. Department of Pathology, Affiliated Hospital of Qingdao University, Qingdao, 266000, Shandong Province, China

    Shuchao Zhao

  4. Department of pathology, Linyi People’s Hospital, Linyi, 276003, Shandong Province, China

    Lizhong Zhang

  5. Department of Neurosurgery, Linyi People’s Hospital, Linyi, 276003, Shandong province, China

    Qimin Song

Authors
  1. Wei Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zongqin Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shuchao Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lizhong Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Qimin Song
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

WC and ZZ are associated in planning the work The experiments were carried out by WC, SZ whereas LZ and QS was also involved in data handling and processing. All the authors contributed equally in preparing the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Qimin Song.

Ethics declarations

Conflict of interest

The authors declare no conflicts of interest.

Human and Animal rights

The animal experiment study protocol was approved by the institute’s committee on animal research, Linyi central hospital, Shandong province, China. The legal and ethical approval was obtained prior to initiation of the research work on animals, and the experiments were performed in accordance with the animal ethical committee guidelines and regulations of Republic of China.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chen, W., Zhao, Z., Zhao, S. et al. Resveratrol and Puerarin loaded polymeric nanoparticles to enhance the chemotherapeutic efficacy in spinal cord injury. Biomed Microdevices 22, 69 (2020). https://doi.org/10.1007/s10544-020-00521-4

Download citation

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s10544-020-00521-4

Keywords

Search

Navigation