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Heterologous expression of a novel Poa pratensis gibberellin 2-oxidase gene, PpGA2ox, caused dwarfism, late flowering, and increased chlorophyll accumulation in Arabidopsis

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Biologia Plantarum

Abstract

Gibberellin 2-oxidases (GA2oxs) irreversibly convert bioactive gibberellins (GAs) and their immediate precursors into inactive GAs via 2-β hydroxylation and so regulate gibberellin content in plants. However, to the best of our knowledge, little has been known about the GA2oxs and its function in cool season turfgrass Poa pratensis. In this study, rapid amplification of cDNA end (RACE) was employed to isolate PpGA2ox from P. pratensis. The open reading frame of PpGA2ox was 1 047 bp in length, corresponding to 348 amino acids. PpGA2ox was localized in both nucleus and cytoplasm. The expression of PpGA2ox could be up-regulated by 10 μM gibberellic acid, 5 μM methyl jasmonate, or 10 μM indole-3-acetic acid. In addition, its native promoter could drive GUS expression in both leaf apex and shoot apical region. Moreover, overexpression of PpGA2ox in Arabidopsis led to GA-deficiency leading to dwarf phenotype, delayed flowering time, and increased chlorophyll content. Our study suggests that PpGA2ox could be a candidate gene for breeding new cultivars of P. pratensis.

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Abbreviations

ABA:

abscisic acid

FLC :

FLOWERING LOCUS C

FT :

FLOWERING LOCUS T

GA:

gibberellin

GA2oxs:

GA2-oxidases

GA3 :

gibberellic acid

GUS:

β-glucuronidase

IAA:

indole-3-acetic acid

MeJA:

methyl jasmonate

MS:

Murashige and Skoog

PAC:

paclobutrazol

qPCR:

quantitative polymerase chain reactions

SOC1 :

SUPPRESSOR OF OVEREXPRESSION OF CO1

WT:

wild type

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Authors and Affiliations

  1. Turfgrass Research Institute, Beijing Forestry University, Beijing, 100083, P.R. China

    P.-H Tan, L. Zhang & S.-X. Yin

  2. Beijing Research and Development Center for Grass and Environment, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, P.R. China

    P.-H Tan & K. Teng

Authors
  1. P.-H Tan
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  2. L. Zhang
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  3. S.-X. Yin
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  4. K. Teng
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Corresponding authors

Correspondence to S.-X. Yin or K. Teng.

Additional information

Acknowledgments: We are very grateful to Dr. Y.-W. Jiang from Department of Agronomy, Purdue University and Miss Z.-S. Sun from School of Foreign Languages, Beihang University for critically reviewing the manuscript. This research was supported by the China Postdoctoral Science Foundation (2017M620677), the Beijing Postdoctoral Research Foundation (No.2017ZZ-087), the Postdoctoral Fund of the Beijing Academy of Agriculture and Forestry Sciences, the National Natural Science Foundation of China (No. 31672477), and the Innovative Project of Beijing Academy of Agriculture and Forestry Sciences (KJCX20151202).

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Tan, PH., Zhang, L., Yin, SX. et al. Heterologous expression of a novel Poa pratensis gibberellin 2-oxidase gene, PpGA2ox, caused dwarfism, late flowering, and increased chlorophyll accumulation in Arabidopsis. Biol Plant 62, 462–470 (2018). https://doi.org/10.1007/s10535-018-0788-1

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  • DOI: https://doi.org/10.1007/s10535-018-0788-1

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