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The effect of boron availability, CO2, and irradiance on relative accumulation of the major boron transport proteins, BOR1 and NIP5;1

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Biologia Plantarum

Abstract

Boron (B) is an essential plant micronutrient. Two major B-transport proteins have been recently identified and partially characterized: BOR1, a high-affinity B efflux transporter involved in xylem loading, and NIP5;1, a plasma-membrane boric-acid channel involved in B uptake. To date, studies of these B transporters have investigated their expression individually (mainly as mRNA), and only in response to variation in B availability (mostly B deficiency); the influence of other factors, such as plant resource status, has not been studied. To address this, we grew geranium (Pelargonium × hortorum cv. Maverick White) plants under ambient or elevated CO2 concentration, different sub-saturating irradiances, and different B availability. For comparison we also grew three other species (Arabidopsis thaliana, Azolla caroliniana, and Hordeum vulgare) under broad range of B supply. Relative accumulation of BOR1 and NIP5;1 proteins were measured using protein-specific antibodies and Western blotting or ELISA. Elevated CO2 significantly increased content of NIP5;1, while increases in irradiance increased BOR1 content, but decreased NIP5;1 content. Across species, content of both transporters often decreased with increasing B availability, but sometimes remained unchanged or even increased, depending on CO2, irradiance, species, or transporter. Content of BOR1 and NIP5;1 was correlated with root proteins, B content, and sugar content (for high CO2 only), as well as B uptake, but CO2 and irradiance often affected these relationships. Thus, relative accumulation of BOR1 and NIP5;1 is influenced not only by B content, as expected, but by other environmental factors as well.

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Abbreviations

BOR1:

boron transporter protein 1

CE:

carboxylation efficiency of photosynthesis

EDTA:

ethylenediaminetetraacetic acid

NIP:

NOD26-like intrinsic proteins

PAR:

photosynthetically active radiation

pNPP:

para-nitrophenylphosphate

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Author notes

  1. J. M. Frantz

    Present address: Pioneer Hi-Bred International, Inc., Johnston, IA, 50131, USA

  2. S. Mishra

    Present address: Department of Biology, Kean University, Union, NJ, 07083, USA

Authors and Affiliations

  1. Department of Environmental Sciences, University of Toledo, Toledo, OH, 43606, USA

    S. Mishra & S. A. Heckathorn

  2. United States Department of Agriculture, Agricultural Research Service, University of Toledo, Toledo, OH, 43606, USA

    J. M. Frantz & C. Krause

Authors
  1. S. Mishra
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  2. S. A. Heckathorn
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  3. J. M. Frantz
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  4. C. Krause
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Corresponding author

Correspondence to S. Mishra.

Additional information

Acknowledgements: This research was supported by the U.S. Department of Agriculture, Agricultural Research Service (SCA 58-3607-9-741 to Scott Heckathorn). The authors thank Douglas Sturtz, Alycia Pittenger, and Russ Friedrich for assistance. Mention of proprietary products or company is included for the reader’s convenience and does not imply any endorsement or preferential treatment by USDA/ARS.

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Mishra, S., Heckathorn, S.A., Frantz, J.M. et al. The effect of boron availability, CO2, and irradiance on relative accumulation of the major boron transport proteins, BOR1 and NIP5;1. Biol Plant 62, 121–128 (2018). https://doi.org/10.1007/s10535-017-0744-5

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  • DOI: https://doi.org/10.1007/s10535-017-0744-5

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