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A germin-like protein gene of rice increased superoxide dismutase activity in transformed tobacco

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Biologia Plantarum

Abstract

Germin and germin-like proteins (GLPs) are a broad and diverse family of developmentally regulated proteins widely distributed in plants. Oryza sativa L. harbours a large family of GLPs and serves as a good model for their study. In the present study, a germin-like protein gene (OsRGLP1) of rice origin was characterized by its heterologous expression in tobacco. The real-time PCR established almost a uniform expression of OsRGLP1 in leaves, stem, and roots of T1 Nicotiana tabacum cv. Samsun. Although no morphological difference was apparent between T0 transgenic and wild-type plants, leaves of mature transgenic plants showed necrotic lesions associated with an elevated content of H2O2, which was evidenced by in situ 3,3′-diaminobenzidine staining. A significantly higher activity of heat resistant superoxide dismutase (SOD) was observed in the transgenic plants as compared to the wild-type. The SOD activity in the transgenic plants was insensitive to potassium cyanide and sensitive to H2O2.

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Abbreviations

CTAB:

cetyldimethylammonium bromide

NBT:

nitroblue tetrazolium

OXO:

oxalate oxidase

SOD:

superoxide dismutase

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Authors and Affiliations

  1. Department of Biochemistry, PMAS-Arid Agriculture University, Rawalpindi, 46300, Pakistan

    T. Yasmin, A. Mumtaz, T. Mahmood, M. Z. Hyder & S. M. S. Naqvi

  2. Department of Biosciences, COMSATS Institute of Information Technology, Islamabad, 45550, Pakistan

    T. Yasmin & M. Z. Hyder

  3. Department of Plant Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan

    T. Mahmood

Authors
  1. T. Yasmin
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  2. A. Mumtaz
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  3. T. Mahmood
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  4. M. Z. Hyder
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  5. S. M. S. Naqvi
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Corresponding author

Correspondence to S. M. S. Naqvi.

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Acknowledgments: We are grateful for a financial assistance to S.M.S. Naqvi (grant No. # 01-03-01-19 from the Agricultural Linkage Program of the Pakistan Agricultural Research Council).

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Yasmin, T., Mumtaz, A., Mahmood, T. et al. A germin-like protein gene of rice increased superoxide dismutase activity in transformed tobacco. Biol Plant 59, 456–462 (2015). https://doi.org/10.1007/s10535-015-0524-z

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  • DOI: https://doi.org/10.1007/s10535-015-0524-z

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