Abstract
Late blight of potato, caused by Phytophthora infestans was studied by using a resistant clone of potato on one side and a susceptible clone on the other side. A gene coding putatively for an α-galactosidase has been isolated by mRNA reverse transcription polymerase chain reaction differential display and was shown to be differentially expressed between the resistant and the susceptible clone. α-Galactosidases catalyse the hydrolysis of α-1,6 linked α-galactose residues from oligosaccharides and it could be shown in the present work that raffinose content decreases at 30 h after infection by P. infestans in the resistant clone.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- EST:
-
expressed sequence tag
- f.m.:
-
fresh mass
- ORF:
-
open reading frame
- QTL:
-
quantitative trait locus
- RT-PCR:
-
reverse transcription polymerase chain reaction
References
Colon, L.T., Budding, D.J., Keizer, L.C.P., Pieters, M.: Components of resistance to late blight (Phytophthora infestans) in eight South American Solanum species.-Eur. J. Plant Pathol. 101: 441–456, 1995.
Doyle, J.J., Doyle, J.L.: A rapid DNA isolation procedure for small quantities of fresh leaf tissue.-Phytochem. Bull. 19: 11–15, 1987.
Evers, D., Dommes, J., Hausman, J.F.: Carbohydrates and resistance to Phytophthora infestans in potato plants.-Acta Physiol. Plant. 25: 171–178, 2003b.
Evers, D., Ghislain, M., Hausman, J.F., Dommes, J.: Differential gene expression in two potato lines differing in their resistance to Phytophthora infestans.-J. Plant Physiol. 160: 709–712, 2003a.
Ghislain, M., Trognitz, B., Herrera, M.R., Solis, J., Casallo, G., Vasquez, C., Hurtado, O., Castillo, R., Portal, L., Orrillo, M.: Genetic loci associated with field resistance to late blight in offspring of Solanum phureja and S. tuberosum grown under short-day conditions.-Theor. appl. Genet. 103: 433–442, 2001.
Guimaraes, V.M., De Rezende, S.T., Moreira, M.A., De Barros, E.G., Felix, C.R.: Characterization of alpha-galactosidases from germinating soybean seed and their use for hydrolysis of oligosaccharides.-Phytochemistry 58: 63–67, 2001.
Haynes, K.G., Christ, B.J.: Heritability of resistance to foliar late blight in a diploid hybrid potato population of Solanum phureja × Solanum stenotomum.-Plant Breed. 118: 431–434, 1999.
Kevers, C., Gaspar, T.: Vitrification of carnation in vitro: changes in water content, extracellular space, air volume, and ion level.-Physiol. veg. 24: 647–653, 1986.
Krieg, P.A. (ed.): A Laboratory Guide to RNA. Isolation, Analysis and Synthesis.-Wiley-Liss, New York 1996.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue culture.-Physiol. Plant. 15: 473–497, 1962.
Nilsson, B.A.: Component analysis of general resistance to Phytophthora infestans in clones from the Columbian potato collection.-Potato Res. 24: 239–244, 1981.
Pukacka, S., Wojkiewicz, E.: Carbohydrate metabolism in Norway maple and sycamore seeds in relation to desiccation tolerance.-J. Plant Physiol. 159: 273–279, 2002.
Reinhardt, A., Hubbard, T.: Using neural networks for prediction of the subcellular location of proteins.-Nucl. Acids Res. 26: 2230–2236, 1998.
Rezessy-Szaboo J.M., Bujna, E., Hoschke, A.: Effect of different carbon and nitrogen sources on α-galactosidase activity originated from Thermomyces lanuginosus CBS 395.62/B.-Acta Aliment. 31: 73–82, 2002.
Sagerstrom, C.G., Sive, H.L.: RNA blot analysis.-In: Krieg, P.A. (ed.): A Laboratory Guide to RNA. Isolation, Analysis, and Synthesis. Pp. 83–103. Wiley-Liss, New York 1996.
Stano, J., Micieta, K., Kovacs, P., Neubert, K., Tintemann, H., Tribulova, N.: Demonstration of activity of α-galactosidase secreted by Cucumis sativus L. cells.-Acta biotechnol. 21: 83–87, 2001.
Trognitz, B.R., Orrillo, M., Portal, L., Roman, C., Ramon, P., Perez, S., Chacon, G.: Evaluation and analysis of reduction of late blight disease in a diploid potato progeny.-Plant Pathol. 50: 281–291, 2001.
Trognitz, F., Manosalva, P., Gysin, R., Nino-Liu, D., Simon, R., Herrera, M. del R., Trognitz, B., Ghislain, M., Nelson, R.: Plant defence genes associated with quantitative resistance to potato late blight in Solanum phureja × dihaploid S. tuberosum hybrids.-Mol. Plant Microbe Interact. 15: 587–597, 2002.
Von Heijne, G.: A new method for predicting signal sequence cleavage sites.-Nucl. Acids Res. 14: 4683–4690, 1986.
Weising, K., Nybom, H., Wolff, K., Meyer, W.: DNA Fingerprinting in Plants and Fungi.-CRC Press, Boca Raton 1995.
Wilson, R., Cataldo, A., Anderson, C.P.: Determination of total non-structural carbohydrates in tree species by high-performance anion-exchange chromatography with pulsed amperometric detection.-Can. J. Forest Res. 25: 2022–2028, 1995.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Evers, D., Ghislain, M., Hoffmann, L. et al. A late blight resistant potato plant overexpresses a gene coding for α-galactosidase upon infection by Phytophthora infestans . Biol Plant 50, 265–271 (2006). https://doi.org/10.1007/s10535-006-0017-1
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10535-006-0017-1