Abstract
Vinasse from the distillation of winemaking residues is a wastewater characterized by high levels of aromatic compounds. Batch cultures of Phlebia rufa showed a significant (p < 0.05) correlation between laccase activity and initial vinasse concentration. The pattern of biodegradation of hydroxybenzoic acids, hydroxycinnamic acids and flavonoids, assessed by HPLC-DAD, revealed that p-hydroxybenzoic acid is the most recalcitrant compound. Vinasse-induced laccase showed electrophoretic homogeneity and molecular weight of 62 kDa after being purified 21-fold. Optimum pH for oxidation of 2,6-dimethoxyphenol (2,6-DMP) was 3.5 and optimum temperature was 50 °C, with an activation energy of 42.8 kJ mol−1. Catalytic efficiency of 2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) oxidation is about two orders of magnitude higher than 2,6-DMP oxidation, being their Km values 36.2 ± 2.6 μM and 303.0 ± 44.7 μM, respectively and kcat values 486.1 s−1 and 179.6 s−1, respectively. Akaike information criterion and Akaike weights were used to discriminate inhibition models that best fitted 2,6-DMP oxidation in the presence of inhibitors. Inhibition constants of mixed-type inhibitors azide and fluoride, and competitive-type inhibitor chloride, showed the following inhibitors potency: azide > fluoride > chloride. Taken together, this study is consistent with the assumption that P. rufa could be a useful tool for aerobic degradation of phenolic-rich wastewaters.
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Acknowledgements
J.M.C.F. is recipient of a fellowship supported by INNOVINE&WINE - Innovation Platform of Vine and Wine, Project NORTE-01-0145-FEDER-000038, co-funded by FEDER/COMPETE/POCI under Project POCI-01-0145-FEDER-006958; this work was also supported by FCT - Portuguese Foundation for Science and Technology under project UIDB/04033/2020.
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Fernandes, J.M.C., Fraga, I., Bezerra, R.M.F. et al. Removal pattern of vinasse phenolics by Phlebia rufa, characterization of an induced laccase and inhibition kinetics modeling. Biodegradation 32, 287–298 (2021). https://doi.org/10.1007/s10532-021-09936-z
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DOI: https://doi.org/10.1007/s10532-021-09936-z