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Simultaneous nitrification–denitrification and microbial community profile in an oxygen-limiting intermittent aeration SBBR with biodegradable carriers

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Abstract

To enhance the startup and efficient simultaneous nitrification and denitrification for sewage treatment, sequencing batch biofilm reactors (SBBRs) partially coupled with rice husk were established and operated under various intermittent micro-aeration cycles (IMCs) and COD/N ratios under oxygen-limiting intermittent aeration conditions. Experimental results showed that the increase of IMCs with non-aeration/micro-aeration mode of (8 h/4 h)1 to (2 h/1 h)4 in a 12 h-cycle accelerated the startup performance and improved NH4+–N and COD removal. NH4+–N, TN and COD removal efficiencies were 98.7 ± 0.9, 89.2 ± 5.2 and 82.9 ± 6.7% at COD/N ratio of 7.6 with the highest IMCs in SBBR, respectively. Higher TN removal efficiencies of 87.2 ± 4.0 and 58.1 ± 3.5% were also achieved at lower COD/N ratio of 5.6 and 2.8, respectively. In SBBRs with various IMCs, facultative denitrifier like genus Acinetobacter and solid-phase denitrifier belonging to Comamonadaceae family were enriched. However, aerobic denitrifiers with function of heterotrophic nitrification like Paracoccus were favored to enrich under higher IMCs condition, and more anoxic denitrifiers like sulfur-based autotrophic denitrifier Thiothrix and heterotrophic denitrifiers like Pseudomonas and Methyloversatilis were observed at lower IMCs condition. Autotrophic nitrifier (Nitrosomonas and Nitrosipra) and heterotrophic nitrifiers both contributed to the efficient nitrification.

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Acknowledgements

This research was supported by Zhejiang Provincial Natural Science Foundation, China (No. LQ15E080012) and Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province, China (No. 2017ZJSHKF05).

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Authors and Affiliations

  1. College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan, 316022, People’s Republic of China

    Lijuan Feng, Rong Jia & Guangfeng Yang

  2. Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province, Hangzhou, People’s Republic of China

    Lijuan Feng & Xiangyang Xu

  3. Zhejiang Design Institute of Water Conservancy & Hydroelectric Power, Hangzhou, 310002, People’s Republic of China

    Zhen Zeng

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  1. Lijuan Feng
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  2. Rong Jia
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  3. Zhen Zeng
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  4. Guangfeng Yang
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  5. Xiangyang Xu
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Correspondence to Lijuan Feng or Guangfeng Yang.

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Feng, L., Jia, R., Zeng, Z. et al. Simultaneous nitrification–denitrification and microbial community profile in an oxygen-limiting intermittent aeration SBBR with biodegradable carriers. Biodegradation 29, 473–486 (2018). https://doi.org/10.1007/s10532-018-9845-x

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