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Generation of a novel monoclonal antibody against inflammatory biomarker S100A8 using hybridoma technology

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Abstract

Objectives

S100A8 is highly expressed in several inflammatory and oncological conditions. To address the current lack of a reliable and sensitive detection method for S100A8, we generated a monoclonal antibody with a high binding affinity to human S100A8 to enable early disease diagnosis.

Results

A soluble recombinant S100A8 protein with a high yield and purity was produced using Escherichia coli. Next, mice were immunized with recombinant S100A8 to obtain anti-human S100A8 monoclonal antibodies using hybridoma technology. Lastly, the high binding activity of the antibody was confirmed and its sequence was identified.

Conclusions

This method, including the production of antigens and antibodies, will be useful for the generation of hybridoma cell lines that produce anti-S100A8 monoclonal antibodies. Moreover, the sequence information of the antibody can be used to develop a recombinant antibody for use in various research and clinical applications.

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Data availability

All data generated or analyzed during this study are included in this published article.

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Funding

This work was supported by Korea Forest Service (Korea Forestry Promotion Institute) R&D Program for Forest Science Technology (2022417A00-2223-AA02), National Research Foundation of Korea (NRF) grants funded by the Korean government (NRF-2017M3A9B6062989, NRF-2020R1I1A307411712), Korea Medical Device Development Fund (KMDF_PR_20200901_0073) and 2022 Hongik University Research Frontier.

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Authors and Affiliations

  1. Department of Biological and Chemical Engineering, Hongik University, Sejong, 30016, Republic of Korea

    Jong-Pyo Kim, Hanool Yun & Hee-Jin Jeong

  2. BioMAX/N-Bio Institute, Seoul National University, Seoul, 08826, Republic of Korea

    Eun-Jung Kim & Byung-Gee Kim

  3. Department of Chemical Engineering, Soongsil University, Seoul, 06978, Republic of Korea

    Yun-Gon Kim

  4. Department of Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon, 34134, Republic of Korea

    Chang-Soo Lee

  5. School of Biopharmaceutical and Medical Sciences, Sungshin Women’s University, Seoul, 02844, Republic of Korea

    Byoung Joon Ko

  6. School of Chemical and Biological Engineering, Seoul National University, Seoul, 08826, Republic of Korea

    Byung-Gee Kim

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  1. Jong-Pyo Kim
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Contributions

Conceptualization: JPK and HJJ, methodology and analysis: JPK, HY, and EJK, investigation: JPK, HY, EJK, YGK, CSL, BJK, BGK. and HJJ, writing—original draft preparation: JPK and HJJ, writing—review and editing: YGK, CSL, BJK, BGK and HJJ, supervision: HJJ All authors reviewed the manuscript.

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Correspondence to Hee-Jin Jeong.

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Kim, JP., Yun, H., Kim, EJ. et al. Generation of a novel monoclonal antibody against inflammatory biomarker S100A8 using hybridoma technology. Biotechnol Lett 45, 589–600 (2023). https://doi.org/10.1007/s10529-023-03364-0

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  • DOI: https://doi.org/10.1007/s10529-023-03364-0

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