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Characterization of a recombinant sucrose isomerase and its application to enzymatic production of isomaltulose

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Abstract

Objective

To characterize a recombinant isomerase that can catalyze the isomerization of sucrose into isomaltulose and investigate its application for the enzymatic production of isomaltulose.

Results

A sucrose isomerase gene from Erwinia sp. Ejp617 was synthesized and expressed in Escherichia coli BL21(DE3). The enzymatic characterization revealed that the optimal pH and temperature of the purified sucrose isomerase were 6.0 and 40 °C, respectively. The enzyme activity was slightly activated by Mn2+and Mg2+, but partially inhibited by Ca2+, Ba2+, Cu2+, Zn2+ and EDTA. The kinetic parameters of Km and Vmax for sucrose were 69.28 mM and 118.87 U/mg, respectively. The time course showed that 240.9 g/L of isomaltulose was produced from 300 g/L of sucrose, and the yield reached 80.3% after bioreaction for 180 min.

Conclusions

This recombinant enzyme showed excellent capability for biotransforming sucrose to isomaltulose at the substrate concentration of 300 g/L. Further investigations should be carried out focusing on selection of suitable heterologous expression system with the aim to improve its expression level.

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Acknowledgements

This work was supported by the Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang, P. R. China (2018R01014) and the Zhejiang provincial Qianjiang talent project.

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Authors and Affiliations

  1. Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014, People’s Republic of China

    Feng Zhang, Feng Cheng, Dong-Xu Jia, Yue-Hao Gu, Zhi-Qiang Liu & Yu-Guo Zheng

  2. The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang road, Hangzhou, 310014, People’s Republic of China

    Feng Zhang, Feng Cheng, Dong-Xu Jia, Yue-Hao Gu, Zhi-Qiang Liu & Yu-Guo Zheng

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  1. Feng Zhang
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  2. Feng Cheng
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  3. Dong-Xu Jia
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  4. Yue-Hao Gu
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Correspondence to Zhi-Qiang Liu.

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Zhang, F., Cheng, F., Jia, DX. et al. Characterization of a recombinant sucrose isomerase and its application to enzymatic production of isomaltulose. Biotechnol Lett 43, 261–269 (2021). https://doi.org/10.1007/s10529-020-02999-7

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  • DOI: https://doi.org/10.1007/s10529-020-02999-7

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