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Inhibition of nattokinase against the production of poly (γ-glutamic Acid) in Bacillus subtilis natto

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Abstract

Objective

To study the effect of nattokinse (NK) on the synthesis of poly(γ-glutamic acid) (γ-PGA) in Bacillus subtilis natto.

Results

γ-PGA yield significantly decreased as NK was added in the original medium. With the increment of NK dosage, the yield decreased increasingly, but biomass increased instead of decreasing. The fact that cell density triggers the synthesis of γ-PGA is a controversial issue. γ-PGA yield and biomass closely correlate with addition time of NK. The later the addition of NK, the more γ-PGA yield decreased but the more biomass increased. It is concluded that cell hunger is a key factor to trigger the transmission of the cell density signal, and NK may inhibit γ-PGA synthesis by alleviating cell hunger. Besides, NK may reduce γ-PGA yield by degrading extracellular γ-PGA molecules. The study of adding L-glutamate of 0–20 g/L to the original medium showed that low concentration of L-glutamate (less than 5 g/L) could promote the synthesis of NK and γ-PGA, and thus NK may inhibit γ-PGA synthesis through strengthening substrate competition.

Conclusions

NK mainly inhibits γ-PGA synthesis in Bacillus subtilis natto through alleviating cell starvation and strengthening substrate competition, and reduces γ-PGA yield through degrading extracellular γ-PGA molecules.

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Acknowledgements

The authors gratefully acknowledge financial support granted to this work by Natural Science Foundation of Anhui Province (1508085SMC212), Key Project of Provincial Natural Science Research of Colleges and Universities in Anhui Province (KJ2014A025), and National Training Programs of Innovation and Entrepreneurship for Undergraduates (201710363041, 201810363047).

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Authors and Affiliations

  1. College of Biochemical Engineering, Anhui Polytechnic University, Wuhu, Anhui, 241000, People’s Republic of China

    Li Wang, Ning Liu, Chenrui Yu, Junliu Chen, Kangjin Hong, Yipeng Zang, Mengmeng Wang & Guangjun Nie

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  1. Li Wang
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  2. Ning Liu
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  3. Chenrui Yu
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  4. Junliu Chen
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  6. Yipeng Zang
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  7. Mengmeng Wang
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  8. Guangjun Nie
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Correspondence to Guangjun Nie.

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Wang, L., Liu, N., Yu, C. et al. Inhibition of nattokinase against the production of poly (γ-glutamic Acid) in Bacillus subtilis natto. Biotechnol Lett 42, 2285–2291 (2020). https://doi.org/10.1007/s10529-020-02941-x

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