Skip to main content
SpringerLink
Log in

A kinetic model to optimize and direct the dose ratio of Dsz enzymes in the 4S desulfurization pathway in vitro and in vivo

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Objective

To enhance the biodesulfurization rate using a kinetic model that directs the ratio of Dsz enzymes.

Results

This study established a kinetic model that predicted the optimal ratio of Dsz enzymes in the 4S biodesulfurization system to be A:B:C = 1:2:4 and 1:4:2. When BCAD+1A+4B+2C, the conversion rate of dibenzothiophene (DBT) to 2-hydroxybiphenyl (HBP) was close to 100% in vitro. When the gene dose of dszC was increased, the HBP yield of the recombinant strain BL21(DE3)/BCAD + C reached approximately 0.012 mM in vivo, which was approximately 6-fold higher than that of the BCAD strain.

Conclusions

According to the results predicted by the enzyme kinetic model, maintaining higher concentrations of DszC and DszB in the desulfurization system can effectively improve the desulfurization efficiency.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Abbreviations

HDS:

Hydrodesulfurization

BDS:

Biodesulfurization

DBT:

Dibenzothiophene

HBP:

2-Hydroxybiphenyl

DBTO2 :

Dibenzothiophene sulfone

HBPS:

2-(2-Hydroxybiphenyl) 2 sulfinic acid salt

HPLC:

High-performance liquid chromatography

References

  • Abinfuentes A, Mohamed ES, Wang DIC, Prather KLJ (2013) Exploring the mechanism of biocatalyst inhibition in microbial desulfurization. Appl Environ Microbiol 79:7807–7817

    Article  CAS  Google Scholar 

  • Agarwal M, Dikshit PK, Bhasarkar JB, Borah AJ, Moholkar VS (2016) Physical insight into ultrasound-assisted biodesulfurization using free and immobilized cells of Rhodococcus rhodochrous MTCC 3552. Chem Eng J 295:254–267

    Article  CAS  Google Scholar 

  • Calzada J, Heras S, Alcon A, Santos VE, Garciaochoa F (2009) Biodesulfurization of dibenzothiophene (DBT) using Pseudomonas putida CECT 5279: a biocatalyst formulation comparison. Energy Fuels 23:155–166

    Article  CAS  Google Scholar 

  • Dejaloud A, Vahabzadeh F, Habibi A (2017) Ralstonia eutropha as a biocatalyst for desulfurization of dibenzothiophene. Bioprocess Biosyst Eng 40:1–12

    Article  CAS  Google Scholar 

  • Denome SA, Olson ES, Young KD (1993) Identification and cloning of genes involved in specific desulfurization of dibenzothiophene by Rhodococcus sp. strain IGTS8. Appl Environ Microbiol 59:2837–2843

    CAS  PubMed  PubMed Central  Google Scholar 

  • Ferreira P, Sousa SF, Fernandes PA, Ramos MJ (2017) Improving the catalytic power of the DszD enzyme for the biodesulfurization of crude oil and derivatives. Chemistry 23:17231–17241

    Article  CAS  PubMed  Google Scholar 

  • Galán B, Díaz E, García JL (2000) Enhancing desulphurization by engineering a flavin reductase-encoding gene cassette in recombinant biocatalysts. Environ Microbiol 2:687–694

    Article  PubMed  Google Scholar 

  • Gumpf JJ, Idrizi K, Watkins L (2017) Expression, purification, and characterization of codon optimized and mutant variations of DszB from N. asteroides. FASEB J 31:764–767

    Google Scholar 

  • Li L et al (2019) Improved efficiency of the desulfurization of oil sulfur compounds in Escherichia coli using a combination of desensitization engineering and DszC overexpression. ACS Synth Biol 8:1441–1451

    Article  CAS  PubMed  Google Scholar 

  • Martínez I, Mohamed ES, Rozas D, García JL, Díaz E (2016) Engineering synthetic bacterial consortia for enhanced desulfurization and revalorization of oil sulfur compounds. Metab Eng 35:46

    Article  CAS  PubMed  Google Scholar 

  • Martínez I, Elsaid MM, Santos VE, García JL, Garcíaochoa F, Díaz E (2017) Metabolic and process engineering for biodesulfurization in gram-negative bacteria. J Biotechnol 262:47–55

    Article  CAS  PubMed  Google Scholar 

  • Rollin JA et al (2015) High-yield hydrogen production from biomass by in vitro metabolic engineering: mixed sugars coutilization and kinetic modeling. Proc Natl Acad Sci USA 112:4964–4969

    Article  CAS  PubMed  Google Scholar 

  • Santos VE, AlcãN A, Martã­N AB, GãMez E, Garcia-Ochoa F, (2007) Desulfurization of dibenzothiophene using the 4S enzymatic route: influence of operational conditions on initial reaction rates. Biocatalysis 25:286–294

    Article  CAS  Google Scholar 

  • Yu Y, Fursule IA, Mills LC, Englert DL, Berron BJ, Payne CM (2017) CHARMM force field parameters for 2′-hydroxybiphenyl-2-sulfinate, 2-hydroxybiphenyl, and related analogs. J Mol Graph Model 72:32–42

    Article  CAS  PubMed  Google Scholar 

Download references

Supporting information

Supplementary Figure 1. The rate of conversion of DBT to HBP by BL21(DE3)/BADC+C.

Funding

This research was financially supported by the National Natural Science Foundation of China (Grant No. 31470159), the National Science Foundation for Young Scientists of China (Grant No. 31400062).

Author information

Authors and Affiliations

  1. Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China

    Lu Li, Zhijie Guo, Wei Zhang, Xihao Liao, Ying Lin & Shuli Liang

  2. Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China

    Lu Li, Zhijie Guo, Wei Zhang, Xihao Liao, Ying Lin & Shuli Liang

  3. College of Science and Engineering, Jinan University, Guangzhou, 510632, China

    Lei Ye

Authors
  1. Lu Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lei Ye
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhijie Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wei Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xihao Liao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ying Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Shuli Liang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Ying Lin or Shuli Liang.

Ethics declarations

Conflict of interest

The authors declare no competing financial interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 175 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Li, L., Ye, L., Guo, Z. et al. A kinetic model to optimize and direct the dose ratio of Dsz enzymes in the 4S desulfurization pathway in vitro and in vivo. Biotechnol Lett 41, 1333–1341 (2019). https://doi.org/10.1007/s10529-019-02730-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-019-02730-1

Keywords

Search

Navigation