Abstract
Objectives
Microbial biofilms have become one of the most significant causes of nosocomial infections. The aim of this study was to examine the potential quorum sensing inhibitor activities of Lactobacillus rhamnosus GG microcapsules.
Results
Lactobacillus rhamnosus GG microcapsules effectively inhibited initial biofilm formation at a concentration of 2.5 × 108 CFU/mL. Furthermore, the inhibition rate was increased to 79% in the Lactobacillus rhamnosus GG microcapsules group, resulting in a reduction in the biofilm maturation stage. In addition, real-time PCR analysis revealed that the LGG microcapsules can act as effective inhibitors of transcriptional activators of the quorum sensing circuit in E.coli, luxS, lsrK, and lsrR.
Conclusions
Lactobacillus rhamnosus GG microcapsules can effectively inhibit biofilm formation and disturb mature biofilms.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (Grant No. 81601734), Liaoning Provincial Natural Science Foundation of China (Grant No. 20180530062) and a Basic Research Projects Grant of the Key Laboratory of the Education Department of Liaoning Province (Grant No. LZ2015028).
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Supplementary Table 1―PCR primer sequences.
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Song, H., Zhang, J., Qu, J. et al. Lactobacillus rhamnosus GG microcapsules inhibit Escherichia coli biofilm formation in coculture. Biotechnol Lett 41, 1007–1014 (2019). https://doi.org/10.1007/s10529-019-02694-2
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DOI: https://doi.org/10.1007/s10529-019-02694-2